IMX Seminar Series - DNA Bioengineering

Can we construct a cell from non-living matter? In search for answers, bottom-up synthetic biology has successfully encapsulated functional sets of biomolecules inside lipid vesicles, yet a “living” synthetic cell remains unattained. Instead of relying exclusively on biological building blocks, the integration of new tools can be a shortcut towards the assembly of active and eventually fully functional synthetic cells. This is especially apparent when considering recent advances in DNA nanotechnology. DNA nanotechnology allowed us to engineer various functional parts for synthetic cells, which, meanwhile have found diverse applications as biophysical probes in cell biology. Recently, we engineered functional DNA-based mimics of a cytoskeleton. These cytoskeletons are capable of stimuli-responsive reversible assembly, cargo transport and can deform giant unilamellar lipid vesicles (GUVs) from within. We further demonstrate the division of GUVs based on phase separation or spontaneous curvture increase and osmosis rather than the biological building blocks of a cell’s division machinery. We derive a parameter-free analytical model which makes quantitative predictions that we verify experimentally. The osmolarity increase can be triggered by enzymatic reactions or by light-triggered release of caged compounds. Ultimately, by coupling GUV division to their informational content and their function, we aim for a prototype of a synthetic cell capable of evolution.
Bio: since 2019
Independent Max Planck Research Group Leader (W2)
Member of the Excellence Cluster 3D Matter Made to Order
Max Planck Institute for Medical Research, Heidelberg, Germany
2017 – 2019
Marie Skłodowska-Curie Postdoctoral Fellow
Max Planck Institute for Intelligent Systems, Stuttgart, Germany
2013 – 2017
Ph.D. in Physics
Gates Cambridge Fellow, Winton Fellow
Cavendish Laboratory, University of Cambridge, United Kingdom
2012 – 2013
M.Phil. in Physics
Cavendish Laboratory, University of Cambridge, United Kingdom
2009 – 2012
B.Sc. in Physics and Molecular Medicine
University of Erlangen, Germany