Microfluidics and Nanopatterning for Bioanalysis and Tissue Engineering
Event details
| Date | 16.07.2014 |
| Hour | 14:00 |
| Speaker | Prof. David Juncker, McGill University, Montréal, QC (CAN) |
| Location | |
| Category | Conferences - Seminars |
BIOENGINEERING SEMINAR
Abstract:
This presentation will cover recent advances from my lab. I will present (i) capillary microfluidics in Si chip and on thread, and demonstrate basic function and how to build complex circuits, and show that like in electronics, capillary circuits can be assembled from elementary building blocks. Next, I will discuss antibody microarrays and multiplexed immunoassays and explain how vulnerability to cross-reactivity has thwarted efforts to scale up these arrays. I will present the scalable antibody colocalization microarray (ACM) and the snap chip for microarray-to-microarray liquid transfer, and show their use for high sensitivity protein profiling and biomarker discovery for breast cancer in blood. In the second part of the presentation I will present a new framework for cell-surface interactions, choice assays, and migration, with the introduction of sets of standardized reference surfaces and cell-surface affinity curves. New micro- and nanocontact printing techniques will be presented to universally pattern proteins on any surface with nanometer resolution. Results of neuronal haptotaxis on digital nanodot gradients with very high dynamic range will conclude this presentation.
Bio:
I was born in Aarau, a small city close to Zurich, in the German part of Switzerland, and moved to a small town close to Neuchâtel, in the French part of Switzerland at the age of twelve. I went on to study at the Institute of Microtechnology of the French speaking University of Neuchâtel, and received a degree in Electronics–Physics in 1996.
In 1997, I was awarded a one-year traveling fellowship from the Swiss Academy of Engineering Sciences and spent 16 months in Japan, first learning some basic Japanese in the beautiful city of Kanazawa, and then staying 1 year as a visiting scientist at the National Metrology Institute of Japan in Tsukuba, working on a microelectromechanical (MEMS) accelerometer.
In 1999 I moved back to the the German speaking part of Switzerland and studied and worked at the IBM Zurich Research Laboratory until 2004. Between 1999 and 2002 I was studying towards my PhD under the supervision of Dr. Emmanuel Delamarche (at IBM) while my official PhD advisor was Dr. Nico de Rooij from the Institute of Microtechnology at the University of Neuchatel which awarded my PhD in 2002. During this time we developed and used soft lithography for high-resolution surface patterning with proteins and autonomous microfluidics that relied on capillary effects for both powering and controlling the flow of liquid. Between 2002 and 2004 we developed the microfluidic probe for local processing of surfaces and of cells, and which constitutes a central theme of our current research.
From 2004 to 2005, I was a postdoc in the Micro- and Nanosystems group of Dr. Christofer Hierold at the Swiss Federal Institute of Technology in Zurich (ETH). I helped setting up the lab, introduced polymer microfabrication and replication processes for making a wireless implantable strain sensor.
End of 2005, I started as an assistant professor in the Biomedical Engineering Department of McGill University, an English-speaking institution in Montréal, a French-English bilingual city in Canada. In 2011, I was promoted to the rank of associate professor with tenure. in 2006, I was been awarded a Canada Research Chair in Micro- and Nanobioengineeringm which was renewed in 2011. In 2012,I was selected as Young Scientist by the IAP - the global network of science academies - and represented Canada at World Economic Forum, Summer Davos New Champions Meeting, Tianjin, China, Sept 10-15th, 2012.
My current interest are in the exploration of miniaturization and integration in biology and medicine, which includes the conception, engineering and utilization of novel micro and nanotechnologies for manipulating, stimulating and studying oligonucleotides, proteins, cells, and tissues. The emerging field of nanobiotechnology, in a broad sense, is the most exciting to me, and is also key to tackle some of the major challenges in biology and medicine, for example identify novel biomarkers for early disease diagnosis or develop low cost point-of-care diagnostics for multiple disease.
Abstract:
This presentation will cover recent advances from my lab. I will present (i) capillary microfluidics in Si chip and on thread, and demonstrate basic function and how to build complex circuits, and show that like in electronics, capillary circuits can be assembled from elementary building blocks. Next, I will discuss antibody microarrays and multiplexed immunoassays and explain how vulnerability to cross-reactivity has thwarted efforts to scale up these arrays. I will present the scalable antibody colocalization microarray (ACM) and the snap chip for microarray-to-microarray liquid transfer, and show their use for high sensitivity protein profiling and biomarker discovery for breast cancer in blood. In the second part of the presentation I will present a new framework for cell-surface interactions, choice assays, and migration, with the introduction of sets of standardized reference surfaces and cell-surface affinity curves. New micro- and nanocontact printing techniques will be presented to universally pattern proteins on any surface with nanometer resolution. Results of neuronal haptotaxis on digital nanodot gradients with very high dynamic range will conclude this presentation.
Bio:
I was born in Aarau, a small city close to Zurich, in the German part of Switzerland, and moved to a small town close to Neuchâtel, in the French part of Switzerland at the age of twelve. I went on to study at the Institute of Microtechnology of the French speaking University of Neuchâtel, and received a degree in Electronics–Physics in 1996.
In 1997, I was awarded a one-year traveling fellowship from the Swiss Academy of Engineering Sciences and spent 16 months in Japan, first learning some basic Japanese in the beautiful city of Kanazawa, and then staying 1 year as a visiting scientist at the National Metrology Institute of Japan in Tsukuba, working on a microelectromechanical (MEMS) accelerometer.
In 1999 I moved back to the the German speaking part of Switzerland and studied and worked at the IBM Zurich Research Laboratory until 2004. Between 1999 and 2002 I was studying towards my PhD under the supervision of Dr. Emmanuel Delamarche (at IBM) while my official PhD advisor was Dr. Nico de Rooij from the Institute of Microtechnology at the University of Neuchatel which awarded my PhD in 2002. During this time we developed and used soft lithography for high-resolution surface patterning with proteins and autonomous microfluidics that relied on capillary effects for both powering and controlling the flow of liquid. Between 2002 and 2004 we developed the microfluidic probe for local processing of surfaces and of cells, and which constitutes a central theme of our current research.
From 2004 to 2005, I was a postdoc in the Micro- and Nanosystems group of Dr. Christofer Hierold at the Swiss Federal Institute of Technology in Zurich (ETH). I helped setting up the lab, introduced polymer microfabrication and replication processes for making a wireless implantable strain sensor.
End of 2005, I started as an assistant professor in the Biomedical Engineering Department of McGill University, an English-speaking institution in Montréal, a French-English bilingual city in Canada. In 2011, I was promoted to the rank of associate professor with tenure. in 2006, I was been awarded a Canada Research Chair in Micro- and Nanobioengineeringm which was renewed in 2011. In 2012,I was selected as Young Scientist by the IAP - the global network of science academies - and represented Canada at World Economic Forum, Summer Davos New Champions Meeting, Tianjin, China, Sept 10-15th, 2012.
My current interest are in the exploration of miniaturization and integration in biology and medicine, which includes the conception, engineering and utilization of novel micro and nanotechnologies for manipulating, stimulating and studying oligonucleotides, proteins, cells, and tissues. The emerging field of nanobiotechnology, in a broad sense, is the most exciting to me, and is also key to tackle some of the major challenges in biology and medicine, for example identify novel biomarkers for early disease diagnosis or develop low cost point-of-care diagnostics for multiple disease.
Practical information
- Informed public
- Free