Optical methods for rapid diagnosis in microbiology
Event details
| Date | 10.01.2017 |
| Hour | 11:15 |
| Speaker | Pierre R. Marcoux CEA, LETI, Division Microtechnologies for Biology and Healthcare, France |
| Location |
PH L1 503 (Aquarium )
|
| Category | Conferences - Seminars |
Today, rapid detection, identification and antibiotic susceptibility testing of bacteria in microbiological diagnosis is a major issue. Methods relying on the observation of few or single bacteria will allow much faster delivery of clinical information. I will report three methods studied at CEA Grenoble regarding the characterisation of bacterial pathogens:
- Rapid enumeration of bacteria: water-in-oil emulsions were used to encapsulate single E. coli cells into picolitre-sized microreactor droplets. Then their enzymatic activity was monitored individually through time-lapse fluorescence imaging.
- Rapid and non-invasive detection of pathogens: Nanoporous glasses are used to trap volatile byproducts of bacterial metabolism. The trapping of these volatile compounds induces changes of the optical properties of glasses, either absorbance or fluorescence, so that pathogens can be detected in a non-invasive way.
- Rapid and label-free identification of pathogens: Incident laser light on bacterial colonies yields a complex pattern due to optical scattering, either forward or backward. The analysis of these scattergrams through pattern recognition algorithms brings an innovative way of identifying unknown bacteria, in a non-destructive and non-invasive method
- Rapid enumeration of bacteria: water-in-oil emulsions were used to encapsulate single E. coli cells into picolitre-sized microreactor droplets. Then their enzymatic activity was monitored individually through time-lapse fluorescence imaging.
- Rapid and non-invasive detection of pathogens: Nanoporous glasses are used to trap volatile byproducts of bacterial metabolism. The trapping of these volatile compounds induces changes of the optical properties of glasses, either absorbance or fluorescence, so that pathogens can be detected in a non-invasive way.
- Rapid and label-free identification of pathogens: Incident laser light on bacterial colonies yields a complex pattern due to optical scattering, either forward or backward. The analysis of these scattergrams through pattern recognition algorithms brings an innovative way of identifying unknown bacteria, in a non-destructive and non-invasive method
Practical information
- Expert
- Free
Organizer
- Romuald Houdré
Contact
- Romuald Houdré