Conferences - Seminars

03SEP
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  Monday 3 September 2018 12:15 SV1717

Single nanoparticle analytics: from viruses via exosomes to drug carriers

By Prof. Fredrik Höök, Chalmers University, Sweden

DISTINGUISHED LECTURE IN BIOLOGICAL ENGINEERING
(sandwiches served)


Abstract:
Next generation drug-delivery vehicles aimed to carry biological drugs, such as proteins or nucleic acids, are often designed to mimic how natural biological nanoparticles, such as viruses and exosomes, transfer genetic information between cells in vivo. Due to the large heterogeneity of nanoparticles irrespective of whether they are of biological or artificial origin, it is crucially important to advance analytical instrumentation to complement ensemble averaging methods[1] with single nanoparticle analytical approaches. A large set of tools with single-nanoparticle sensitivity is now available, to which we recently contributed a concept that enables simultaneous fluorescent and scattering-based label-free imaging of surface-bound biological nanoparticles [2]. Examples will be shown that illustrate the use of this scattering microscopy concept to i) investigate supported lipid bilayer formation, ii) label-free measurements of protein binding to individual liposomes, iii) characterize DLVO-controlled non-specific interactions at cell-membrane mimics,[3] and detergent free enrichment of pre-defined membrane proteins in crude cell membranes.[4] By using a two dimensional fluid supported lipid bilayer, to which biological nanoparticles are directly anchored and imaged, we have also  developed a new means to simultaneously determine both nanoparticle size and fluorescence / scattering intensity,[5] which may potentially offer flow-cytometry-like sorting based on distinct features of individual nanoparticles. This 2D flow nanometry concept will be discussed in the context of improved characterization of individual biological nanoparticles of diagnostic and therapeutic significance.
References
[1] Rupert, D. et al. Analytical Chemistry 88, 9980 (2016)
[2] Agnarsson, B. A. et al. ACS Nano 9, 11849 (2015).
[3] Lundgren, A. et al. ACS Nano 10, 9974 (2016).
[4] Lundgren, A. et al. Nano Letters 18, 381 (2018)
[5] Block, S. et al. Nature Communication 7, 12956 (2016)

 
Bio:
Fredrik Höök received a PhD in physics from Chalmers, Sweden, in 1997 and his research has focused on the development of surface-based bioanalytical tools, such as QCM-D, localized SPR and more recently TIRF microscopy, and their combination with microfluidics for fundamental studies of cell-membrane mimics but also for use in diagnostic and drug-screening applications. Efforts are presently focused on (i) new methods with single molecule sensitivity for studies of membrane proteins and virus infection, (ii) microfluidic platforms for cell-membrane chromatography and (iii) sophisticated surface modifications and nanofabrication schemes to facilitate improved analysis of supported cell-membrane mimics, membrane proteins and virus binding and infection. He is co-founder of Q-Sense, www.q-sense.com, who is a lead developer of QCM-D instruments. He has been awarded an Individual Grant for the Advancement of Research Leaders by the Swedish Foundation for Strategic Research in 2006 and the Göran Gustafsson prize in Physics by the Royal Swedish Society of Academy in 2012.

Organization Prof. Hatice Altug

Contact Institute of Bioengineering (IBI, Christina Mattsson)

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