SPR biosensing using graphene-based chips
Event details
| Date | 31.05.2016 |
| Hour | 10:00 |
| Speaker |
Dr. Yury Stebunov Moscow Institute of Physics and Technology, Russia |
| Location |
BSP 318
|
| Category | Conferences - Seminars |
Graphene and graphene oxide are opening up many new opportunities for biosensing
applications. The hexagonal lattice structure of graphene and its derivatives allows its interaction
with a wide range of biological substances via pi-stacking. In addition, graphene oxide possesses
different oxygen-containing functional groups for the covalent immobilization of biomolecules.
Moreover, reduction of graphene oxide can finely tune chemical, electrical and optical properties of
carbon material for specific biosensing applications. The major advantage of graphene and its
derivatives for biosensing applications is the extremely high surface area of different structures
made from these materials, which provides high immobilization efficiency for a wide range of
biologically significant substances such as DNAs, RNAs, proteins, including antibodies and
membrane proteins, viruses, and bacteria. Here, we describe a novel type of graphene oxide
linking layer for highly sensitive biosensing based on surface plasmon resonance (SPR), which
has become an indispensable tool for scientific research and drug development [1]. During the last
three decades, researchers have used only two technologies of linking layers for SPR biosensors,
which are based on self-assembled monolayers of thiol molecules and on hydrogel layers. Using
graphene and its derivatives, we developed biosensor chips for existing commercial biosensors,
whose sensitivity is higher than for commercial sensor chips available on the market [2] (Fig. 1-2).
Modification of carboxyl groups to N-hydroxysuccinimide esters in the flow cell of SPR biosensor
demonstrated that the number of carboxyl groups, which can be used for molecule immobilization,
is more than 20 times higher in the graphene oxide linking layer than in the linking layer of
commercial hydrogel-based sensor chip. In addition, the graphene oxide sensor chip was
demonstrated to be 3 times more sensitive comparing to the commercial hydrogel chips when
using in the standard biosensing protocol based on streptavidin-biotin interaction with streptavidin
immobilized on the GO surface via pi-stacking. In conclusion, SPR biosensor chips based on
graphene and its derivatives have higher sensitivity comparing to commercially available chips
based on hydrogels. This will enable us to investigate interactions of protein targets with small
ligands and will broad-en and accelerate academic and pharmaceutical research.
[1] Y.V. Stebunov, O.A. Aftenieva, A.V. Arsenin, V.S. Volkov, ACS Appl. Mat. Interfaces 7, 21727-
21734 (2015).
[2] A.V. Arsenin, Yu.V. Stebunov. RU Patent Application No. 2527699 (Feb 2013); US Patent
Application No. 20150301039 (Oct 2015).
applications. The hexagonal lattice structure of graphene and its derivatives allows its interaction
with a wide range of biological substances via pi-stacking. In addition, graphene oxide possesses
different oxygen-containing functional groups for the covalent immobilization of biomolecules.
Moreover, reduction of graphene oxide can finely tune chemical, electrical and optical properties of
carbon material for specific biosensing applications. The major advantage of graphene and its
derivatives for biosensing applications is the extremely high surface area of different structures
made from these materials, which provides high immobilization efficiency for a wide range of
biologically significant substances such as DNAs, RNAs, proteins, including antibodies and
membrane proteins, viruses, and bacteria. Here, we describe a novel type of graphene oxide
linking layer for highly sensitive biosensing based on surface plasmon resonance (SPR), which
has become an indispensable tool for scientific research and drug development [1]. During the last
three decades, researchers have used only two technologies of linking layers for SPR biosensors,
which are based on self-assembled monolayers of thiol molecules and on hydrogel layers. Using
graphene and its derivatives, we developed biosensor chips for existing commercial biosensors,
whose sensitivity is higher than for commercial sensor chips available on the market [2] (Fig. 1-2).
Modification of carboxyl groups to N-hydroxysuccinimide esters in the flow cell of SPR biosensor
demonstrated that the number of carboxyl groups, which can be used for molecule immobilization,
is more than 20 times higher in the graphene oxide linking layer than in the linking layer of
commercial hydrogel-based sensor chip. In addition, the graphene oxide sensor chip was
demonstrated to be 3 times more sensitive comparing to the commercial hydrogel chips when
using in the standard biosensing protocol based on streptavidin-biotin interaction with streptavidin
immobilized on the GO surface via pi-stacking. In conclusion, SPR biosensor chips based on
graphene and its derivatives have higher sensitivity comparing to commercially available chips
based on hydrogels. This will enable us to investigate interactions of protein targets with small
ligands and will broad-en and accelerate academic and pharmaceutical research.
[1] Y.V. Stebunov, O.A. Aftenieva, A.V. Arsenin, V.S. Volkov, ACS Appl. Mat. Interfaces 7, 21727-
21734 (2015).
[2] A.V. Arsenin, Yu.V. Stebunov. RU Patent Application No. 2527699 (Feb 2013); US Patent
Application No. 20150301039 (Oct 2015).
Practical information
- Expert
- Free
Organizer
- Prof. Giovanni Dietler