Structure of membrane proteins in native membranes by cryo-EM
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Event details
Date | 29.07.2024 |
Hour | 14:15 |
Speaker |
Prof. Mikhail Kudryashev, Max Delbrück Center for Molecular Medicine and Institute of Medical Physics and Biophysics Charité – Universitätsmedizin, Berlin, Germany |
Location | |
Category | Conferences - Seminars |
Event Language | English |
Talk by Prof. Mikhail Kudryashev from Max Delbrück Center for Molecular Medicine in the Helmholtz Association
Subtomogram averaging from cryo electron tomograms is a powerful method to determine structures of macromolecules in their native state. Outstanding applications to protein lattices, coats and ribosomes provided unique insights into their functions and even revealed interactions with small molecules in situ. For other macromolecules, such as membrane proteins, which are present in tomograms in limited numbers, the throughput of data processing and the processing time are key bottlenecks in obtaining high-resolution reconstructions. This is particularly the case for membrane proteins that are typically present in tomograms in moderate amounts.
I will introduce the tools that we developed in our lab for in situ structural biology with a focus on a large ion channel RyR1 which is a part of the excitation-contraction coupling in muscle. TomoBEAR is a workflow for processing of tomographic data utilizing common cryo-EM tools and original code that allows transparent near-automated tomographic pre-processing, alignment, reconstruction and particle identification followed by structural analysis.
In the second part of the talk I will show our recent results on understanding molecular architecture of synaptic vesicles. We imaged neurons grown on EM grids and purified synaptic vesicles by cryo electron tomography. We could identify individual proteins important for the function of synaptic vesicles.
The speaker: Misha Kudryashev, Group Leader - In Situ Structural Biology at Max Delbrück Center for Molecular Medicine in the Helmholtz Association in Berlin, Germany
and Professor for In Situ Structural Biology at the Institute of Medical Physics and Biophysics, Charité – Universitätsmedizin, Berlin, Germany
Practical information
- Informed public
- Free