Writing and Reading DNA Methylation
Event details
| Date | 01.07.2013 |
| Hour | 12:15 › 13:15 |
| Speaker |
Prof. Dirk Schübeler, Friedrich Miescher Institute for Biomedical Research, Basel (CH) Bio: 1998 PhD, Technical University, Braunschweig, Germany 1995 MSc, Technical University, Braunschweig, Germany Positions held 2008- Senior Group Leader, Friedrich Miescher Institute for Biomedical Research, Basel 2003-2007 Junior Group Leader, Friedrich Miescher Institute for Biomedical Research, Basel 1998-2003 Postdoctoral Fellow, Fred Hutchinson Cancer Research Center, Seattle Honours 2012 Election to Academia Europaea 2011 Adjunct Professor, University of Basel 2009 Member, EMBO 2009 Privatdozent, University of Basel 2008 European Research Council Starting Independent Researcher Award 2007 Friedrich Miescher Prize, Swiss Society for Biochemistry 2006 EMBO Young Investigator Award 2006 Member, Epigenome Network of Excellence 2000-2002 Postdoctoral Fellowship, Rett Syndrome Research Foundation 1998-2000 Postdoctoral Fellowship, Deutsche Forschungsgemeinschaft 1997 Thesis award, GBF Förderpreis 1995-1998 Graduate Fellowship, Foundation of the German Chemical Industry |
| Location | |
| Category | Conferences - Seminars |
Abstract:
Chromatin and DNA modifications have emerged as a critical component for gene regulation in higher eukaryotes. Yet how these epigenetic variables are targeted to specific sites of the genome and how they influence cellular potential and identity is still poorly understood.
We have generated global maps of DNA methylation, histone modifications and replication in higher eukaryotes using stem cell differentiation as a dynamic cellular model for pluripotency, lineage commitment and terminal differentiation.
This analysis allowed us to identify genomic sites that change their epigenetic status cell-state specific. Based on the resulting datasets we generate models how these epigenetic variables are targeted, which we test by genetic perturbation of involved modifiers and mutation of putative recruiting elements. In order to systematically define how chromatin components are targeted to the genome we have combined epitope tagging with genome editing enabling us to study the genomic location of a family of proteins that are putative readers of DNA methylation.
Our results suggest that the actual DNA sequence of regulatory regions is a key determinant of their DNA methylation and chromatin state, a finding, which will be discussed in the light of current models of the function of epigenetic restriction during development.
Relevant recent publications:
- Tuncay Baubec, Robert Ivanek, Florian Lienert and Dirk Schübeler. (2013) Methylation-dependent and -independent genomic targeting principles of the MBD protein family, Cell, 153, 480–492
- Michael B. Stadler, Rabih Murr, Lukas Burger, Robert Ivanek, Florian Lienert, Anne Schöler, Christiane Wirbelauer, Edward J. Oakeley, Dimos Gaidatzis, Vijay K. Tiwari and Dirk Schübeler. (2011) DNA binding factors shape the mouse methylome at distal regulatory regions, Nature, 480, 490-495
- Florian Lienert, Christiane Wirbelauer, Indrani Som, Ann Dean, Fabio Mohn and Dirk Schübeler. (2011) Identification of genetic elements that autonomously determine DNA methylation states, Nature Genetics, 43, 1091-97
Chromatin and DNA modifications have emerged as a critical component for gene regulation in higher eukaryotes. Yet how these epigenetic variables are targeted to specific sites of the genome and how they influence cellular potential and identity is still poorly understood.
We have generated global maps of DNA methylation, histone modifications and replication in higher eukaryotes using stem cell differentiation as a dynamic cellular model for pluripotency, lineage commitment and terminal differentiation.
This analysis allowed us to identify genomic sites that change their epigenetic status cell-state specific. Based on the resulting datasets we generate models how these epigenetic variables are targeted, which we test by genetic perturbation of involved modifiers and mutation of putative recruiting elements. In order to systematically define how chromatin components are targeted to the genome we have combined epitope tagging with genome editing enabling us to study the genomic location of a family of proteins that are putative readers of DNA methylation.
Our results suggest that the actual DNA sequence of regulatory regions is a key determinant of their DNA methylation and chromatin state, a finding, which will be discussed in the light of current models of the function of epigenetic restriction during development.
Relevant recent publications:
- Tuncay Baubec, Robert Ivanek, Florian Lienert and Dirk Schübeler. (2013) Methylation-dependent and -independent genomic targeting principles of the MBD protein family, Cell, 153, 480–492
- Michael B. Stadler, Rabih Murr, Lukas Burger, Robert Ivanek, Florian Lienert, Anne Schöler, Christiane Wirbelauer, Edward J. Oakeley, Dimos Gaidatzis, Vijay K. Tiwari and Dirk Schübeler. (2011) DNA binding factors shape the mouse methylome at distal regulatory regions, Nature, 480, 490-495
- Florian Lienert, Christiane Wirbelauer, Indrani Som, Ann Dean, Fabio Mohn and Dirk Schübeler. (2011) Identification of genetic elements that autonomously determine DNA methylation states, Nature Genetics, 43, 1091-97
Links
Practical information
- Informed public
- Free
- This event is internal
Organizer
- Prof. Bart Deplancke