Advances in Imaging Fluorescence Correlations Spectroscopy
Event details
| Date | 02.09.2013 |
| Hour | 16:00 › 17:00 |
| Speaker | Prof. Thorsten WOHLAND, Dept of Biological Sciences and Chemistry, National University of Singapore |
| Location |
CH B3 30
|
| Category | Conferences - Seminars |
Imaging Fluorescence Correlation Spectroscopy (Imaging FCS) has emerged as a powerful technique to multiplex FCS and allow the recording of FCS images. This provides a number of distinct advantages: 1) One can collect thousands of correlation functions simultaneously resulting in excellent statistics. 2) The imaging format contains spatio-temporal information which provides insights into structure and dynamics of the sample. In particular the FCS diffusion laws, which can provide information of the structure of a sample beyond the optical resolution limit, are simple to implement within Imaging FCS. 3) Light sheet based illumination schemes require a much lower light exposure per measurement and thus allow more measurements to be collected per sample. 4) The imaging mode in time allows the recording of FCS time lapse videos to observe changes in molecular dynamics over time. Here we demonstrate the capabilities of imaging FCS in 2D and 3D using total internal reflection (TIR) and single plane illumination microscopy (SPIM), as illumination modes, respectively. We investigate the membrane active human Islet Amyloid Polypeptide (hIAPP) on cell membranes using imaging TIR-FCS, and investigate molecular interactions in 3D by SPIM-FCS and FCCS. These measurements demonstrate that Imaging FCS can provide quantitative images of biological samples
Practical information
- General public
- Free
Organizer
- Prof. Horst Vogel, LCPPM
Contact
- Verena Tabet