Biophysical Aspects of T Cell Activation
Event details
Date | 20.05.2019 |
Hour | 12:15 |
Speaker | Prof. Claire Hivroz, Institut Curie, Paris (F) |
Location | |
Category | Conferences - Seminars |
WEEKLY BIOENGINEERING COLLOQUIA SERIES
(sandwiches served)
Abstract:
When a migrating T cell contacts an antigen presenting cell (APC), and when it forms a radially symmetric mature immune synapse (IS), multiple forces are exerted on the molecules involved in the contacts between the two cells. Hence, molecular contacts between TCR and pMHC, integrins and their ligands, and costimulatory molecules and their ligands are submitted to constant strain. These forces can change the kinetics of association/dissociation between the receptors and their ligands and induce conformational changes that might induce signaling cascades. Studies from others and us have shown that the generation of molecular forces at the IS is induced by TCR triggering and that T cells respond differently depending on the mechanical properties of the stimulatory surfaces they encounter. This corresponds to an adaptation of the forces generated at the IS to the substrate stiffness. These evidences suggest that mechanical force is integrally involved in T cell activation. We will discuss 1) how TCR triggering induces forces that can adapt to the rigidity of the substrates. 2) the viscoelastic properties of myeloid APCs and their regulation by inflammation. 3) how the viscoelastic properties of the environment can modify the functional program of T lymphocytes.
Publications on the subject:
Bio:
Since 2002: Director of Research at the INSERM (DR2)
Since 1998: Team leader (INSERM U932, Immunité et Cancer) at the Curie Institute, Paris, F
1991-1998: Postdoctoral position, then team leader in INSERM U429, Hôpital Necker, Paris, F
1989-1991: Postdoctoral position at Imperial Cancer Research Fund., London, UK
1985-1989: PhD student, Hôpital Saint-Louis, Paris, F; PhD from University Paris 7
1985: D.E.A. from University Paris 7, Hôpital Saint Louis, Paris, F
Zoom link for attending remotely: https://epfl.zoom.us/j/249502771
(sandwiches served)
Abstract:
When a migrating T cell contacts an antigen presenting cell (APC), and when it forms a radially symmetric mature immune synapse (IS), multiple forces are exerted on the molecules involved in the contacts between the two cells. Hence, molecular contacts between TCR and pMHC, integrins and their ligands, and costimulatory molecules and their ligands are submitted to constant strain. These forces can change the kinetics of association/dissociation between the receptors and their ligands and induce conformational changes that might induce signaling cascades. Studies from others and us have shown that the generation of molecular forces at the IS is induced by TCR triggering and that T cells respond differently depending on the mechanical properties of the stimulatory surfaces they encounter. This corresponds to an adaptation of the forces generated at the IS to the substrate stiffness. These evidences suggest that mechanical force is integrally involved in T cell activation. We will discuss 1) how TCR triggering induces forces that can adapt to the rigidity of the substrates. 2) the viscoelastic properties of myeloid APCs and their regulation by inflammation. 3) how the viscoelastic properties of the environment can modify the functional program of T lymphocytes.
Publications on the subject:
- Sawicka A, Babataheri A, Dogniaux S, Barakat AI, Gonzalez-Rodriguez D, Hivroz C*, Husson J*. 2017. Micropipette force probe to quantify single-cell force generation: application to T-cell activation. Mol Biol Cell 28: 3229-39. * Co-corresponding authors.
- Saitakis M, Dogniaux S, Goudot C, Bufi N, Asnacios S, Maurin M, Randriamampita C, Asnacios A*, Hivroz C*. 2017. Different TCR-induced T lymphocyte responses are potentiated by stiffness with variable sensitivity. Elife 6. * Co-corresponding authors.
- Hivroz C, Saitakis M. 2016. Biophysical Aspects of T Lymphocyte Activation at the Immune Synapse. Front Immunol 7: 46
- Bufi N, Saitakis M, Dogniaux S, Buschinger O, Bohineust A, Richert A, Maurin M, Hivroz C*, Asnacios A*. 2015. Human Primary Immune Cells Exhibit Distinct Mechanical Properties that Are Modified by Inflammation. Biophys J 108: 2181-90. * Co-corresponding authors.
- Husson J, Chemin K, Bohineust A, Hivroz C*, Henry N*. 2011. Force generation upon T cell receptor engagement. PLoS ONE 6: e19680. * Co-corresponding authors.
Bio:
Since 2002: Director of Research at the INSERM (DR2)
Since 1998: Team leader (INSERM U932, Immunité et Cancer) at the Curie Institute, Paris, F
1991-1998: Postdoctoral position, then team leader in INSERM U429, Hôpital Necker, Paris, F
1989-1991: Postdoctoral position at Imperial Cancer Research Fund., London, UK
1985-1989: PhD student, Hôpital Saint-Louis, Paris, F; PhD from University Paris 7
1985: D.E.A. from University Paris 7, Hôpital Saint Louis, Paris, F
Zoom link for attending remotely: https://epfl.zoom.us/j/249502771
Practical information
- Informed public
- Free
Organizer
Contact
- Institute of Bioengineering (IBI), Christina Mattsson