ChemBio seminar by Prof. Chayasith Uttamapinant (VISTEC) - CH-635
Event details
| Date | 30.05.2023 |
| Hour | 16:15 › 17:15 |
| Speaker | Prof. Chayasith Uttamapinant |
| Location | |
| Category | Conferences - Seminars |
| Event Language | English |
Title: Engineering sustainable resources for CRISPR-based diagnosis of infectious diseases
Abstract:
I will discuss our development of CRISPR-based diagnostics for infectious diseases toward point-of-care (POC) use outside of labs. We have developed a robust multiplexed CRISPR-based detection using orthogonal CRISPR-associated nucleases to simultaneously diagnose SARS-CoV-2 infection and pinpoint the causative SARS-CoV-2 variant of concern (VOC)—including recent globally dominant VOCs Delta (B.1.617.2) and Omicron (B.1.1.529) and have implemented the CRISPR-based test technology for COVID-19 diagnosis, with the approval of the FDA of Thailand. The platform has features for POC use: premixed freeze-dried reagents for easy use and storage; convenient smartphone-based readouts; and a one-pot variant of the multiplexed detection. Beyond COVID-19, our team and collaborators are applying CRISPR-based diagnostics to neglected fatal tropical diseases, whose diagnosis is challenging yet critically important for timely treatment. To reduce reliance on proprietary reagents and enable sustainable use of the technology in low- and middle-income countries, we locally produce a complex recombinase polymerase isothermal amplification (RPA) reaction typically used in conjunction with CRISPR-based detection and demonstrate its equivalent efficiency to commercial counterparts. The ability to produce RPA in house enables us to probe the phase separation-dependent amplification of RPA and engineer its bottleneck components, further improving the reaction for multiplexed diagnosis.
Speaker's biography:
Tao Uttamapinant received his AB in Chemistry from Harvard College in 2007. He completed his PhD in biological chemistry with Prof. Alice Ting at MIT in 2013, then trained with Prof. Jason Chin as a Marie Curie International Incoming and Career Development Fellow at Medical Research Council Laboratory of Molecular Biology from 2013-2018. Tao started his faculty appointment at VISTEC, Thailand in late 2018. Research in his laboratory focuses on the development of new tools to characterize locally synthesized proteins and proteoforms; plastic bioremediation; and open-source molecular diagnostic tools.
Lab website: https://www.vistec.ac.th/academic/faculty_detail.php?school=BSE&id=83
Updated publications: https://scholar.google.co.uk/citations?user=uUliPp0AAAAJ&hl=en
Abstract:
I will discuss our development of CRISPR-based diagnostics for infectious diseases toward point-of-care (POC) use outside of labs. We have developed a robust multiplexed CRISPR-based detection using orthogonal CRISPR-associated nucleases to simultaneously diagnose SARS-CoV-2 infection and pinpoint the causative SARS-CoV-2 variant of concern (VOC)—including recent globally dominant VOCs Delta (B.1.617.2) and Omicron (B.1.1.529) and have implemented the CRISPR-based test technology for COVID-19 diagnosis, with the approval of the FDA of Thailand. The platform has features for POC use: premixed freeze-dried reagents for easy use and storage; convenient smartphone-based readouts; and a one-pot variant of the multiplexed detection. Beyond COVID-19, our team and collaborators are applying CRISPR-based diagnostics to neglected fatal tropical diseases, whose diagnosis is challenging yet critically important for timely treatment. To reduce reliance on proprietary reagents and enable sustainable use of the technology in low- and middle-income countries, we locally produce a complex recombinase polymerase isothermal amplification (RPA) reaction typically used in conjunction with CRISPR-based detection and demonstrate its equivalent efficiency to commercial counterparts. The ability to produce RPA in house enables us to probe the phase separation-dependent amplification of RPA and engineer its bottleneck components, further improving the reaction for multiplexed diagnosis.
Speaker's biography:
Tao Uttamapinant received his AB in Chemistry from Harvard College in 2007. He completed his PhD in biological chemistry with Prof. Alice Ting at MIT in 2013, then trained with Prof. Jason Chin as a Marie Curie International Incoming and Career Development Fellow at Medical Research Council Laboratory of Molecular Biology from 2013-2018. Tao started his faculty appointment at VISTEC, Thailand in late 2018. Research in his laboratory focuses on the development of new tools to characterize locally synthesized proteins and proteoforms; plastic bioremediation; and open-source molecular diagnostic tools.
Lab website: https://www.vistec.ac.th/academic/faculty_detail.php?school=BSE&id=83
Updated publications: https://scholar.google.co.uk/citations?user=uUliPp0AAAAJ&hl=en
Practical information
- Informed public
- Free