Conformational Dynamics and Diversity of Single G Protein-Coupled Receptors Trapped in Solution or Imaged in Living Cells
Event details
| Date | 10.10.2011 |
| Hour | 07:00 |
| Speaker | Alexandre Fürstenberg, University of Geneva |
| Location |
BM5204
|
| Category | Conferences - Seminars |
We focus on our use of an Anti-Brownian ELectrokinetic (ABEL) trap, a novel device enabling the study of individual fluorescent nanoscale objects in solution without requiring chemical immobilization, to investigate the conformational dynamics of the b2-adrenergic receptor (b2AR), a canonical GPCR involved in smooth muscle regulation. The ABEL trap uses fast electrokinetic feedback in a microfluidic configuration to enable direct observation of single fluorescently-labelled β2AR molecules for hundreds of milliseconds to seconds. By using a conformation-sensitive reporter dye, we observe a diversity of fluorescence intensity and lifetime levels in individual β2AR molecules, indicating changes in radiative lifetime and the existence of a vast range of discrete conformational states. We find that agonist binding increases the dwell times of these states and monitor millisecond fluctuations within these states.
Practical information
- General public
- Free
Contact
- Ubezio Fabienne