EPFL BioE Talks SERIES "Building the Human Lungs: Lessons From Organoids and Gene Editing"
Event details
| Date | 29.04.2024 |
| Hour | 12:15 › 13:15 |
| Speaker | Prof. Emma Rawlins, Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge (UK) |
| Location | Online |
| Category | Conferences - Seminars |
| Event Language | English |
WEEKLY EPFL BIOE TALKS SERIES (sandwiches provided)
Abstract:
Organoids derived from primary human tissues retain key functional characteristics of the tissue of interest and have the potential to improve in vitro assays used for disease modelling and drug development. However, the burgeoning CRISPR toolkits have not yet been widely applied to human organoids. We have developed efficient methodologies for the use of CRISPR techniques in primary human embryonic lung organoids. These will be widely adaptable to other human organoid systems and include homology directed repair, CRISPRi, CRISPRa and drop-out screens. This talk will illustrate how we have applied these systems to develop new human lung organoid models for fundamental developmental biology and disease research. These tools have allowed us to identify new cell states and crucial cell communication events underlying lung morphogenesis, as well as model genetic forms of human lung disease.
Bio:
Emma Rawlins obtained her PhD in developmental biology from the University of Edinburgh in 2002. For her PhD she worked with Dr Andrew Jarman on cell fate specification in the developing Drosophila PNS. She performed postdoctoral work with Prof. Brigid Hogan at Duke University from 2004 – 2009 where she identified stem cell populations in the developing, homeostatic and repairing mouse lungs. In 2009 she started her lab at the Gurdon Institute, University of Cambridge and in 2020 was promoted to Senior Group Leader. Emma is also an Associate Professor in the Department of Physiology, Development and Neuroscience. Her lab works on lung stem and progenitor cell biology, combining innovative human organoid models with mouse genetics.
Zoom link (with one-time registration for the whole series) for attending remotely: https://go.epfl.ch/EPFLBioETalks
Instructions for 1st-year Ph.D. students who are under EDBB’s mandatory seminar attendance rule:
IN CASE you cannot attend in-person in the room, please make sure to
Abstract:
Organoids derived from primary human tissues retain key functional characteristics of the tissue of interest and have the potential to improve in vitro assays used for disease modelling and drug development. However, the burgeoning CRISPR toolkits have not yet been widely applied to human organoids. We have developed efficient methodologies for the use of CRISPR techniques in primary human embryonic lung organoids. These will be widely adaptable to other human organoid systems and include homology directed repair, CRISPRi, CRISPRa and drop-out screens. This talk will illustrate how we have applied these systems to develop new human lung organoid models for fundamental developmental biology and disease research. These tools have allowed us to identify new cell states and crucial cell communication events underlying lung morphogenesis, as well as model genetic forms of human lung disease.
Bio:
Emma Rawlins obtained her PhD in developmental biology from the University of Edinburgh in 2002. For her PhD she worked with Dr Andrew Jarman on cell fate specification in the developing Drosophila PNS. She performed postdoctoral work with Prof. Brigid Hogan at Duke University from 2004 – 2009 where she identified stem cell populations in the developing, homeostatic and repairing mouse lungs. In 2009 she started her lab at the Gurdon Institute, University of Cambridge and in 2020 was promoted to Senior Group Leader. Emma is also an Associate Professor in the Department of Physiology, Development and Neuroscience. Her lab works on lung stem and progenitor cell biology, combining innovative human organoid models with mouse genetics.
Zoom link (with one-time registration for the whole series) for attending remotely: https://go.epfl.ch/EPFLBioETalks
Instructions for 1st-year Ph.D. students who are under EDBB’s mandatory seminar attendance rule:
IN CASE you cannot attend in-person in the room, please make sure to
- send D. Reinhard a note well ahead of time (ideally before seminar day), informing that you plan to attend the talk online, and, during seminar:
- be signed in on Zoom with a recognizable user name (not any alias making it difficult or impossible to identify you).
Practical information
- Informed public
- Registration required
Organizer
- Prof. Alexandre Persat, EPFL
Contact
- Institute of Bioengineering (IBI), Dietrich REINHARD