EPFL BioE Talks SERIES "DNA Origami Coating Strategies for Enhanced Stability and Delivery"
Event details
| Date | 07.12.2020 |
| Hour | 16:00 › 16:30 |
| Speaker | Prof. Mauri A. Kostiainen, Aalto University, Espoo (SF) |
| Location | Online |
| Category | Conferences - Seminars |
WEEKLY EPFL BIOE TALKS SERIES
(note that this talk is number one of a double-feature seminar - see details of the second talk here)
Abstract:
Fully addressable DNA nanostructures, especially DNA origami, possess huge potential to serve as inherently biocompatible and versatile molecular platforms. However, their use as delivery vehicles in therapeutics is compromised by their low stability and poor transfection rates. Our studies have shown that DNA origamis can be coated non-covalently by various materials to tackle the aforementioned issues. In detail, we have demonstrated that coatings utilizing either virus capsid proteins, serum albumin, phthalocyanine dyes or cationic lipids can enhance the delivery, and especially stability of the structures. The electrostatic complexation strategy is highly modular and suitable for a wide range of DNA-based templates.
References:
Mikkilä, J.; Eskelinen, A.-P.; Niemelä, E.; Linko, V.; Frilander, M.; Törmä, P.; Kostiainen, M. A. Virus Encapsulated DNA Origami Nanostructures for Cellular Delivery, Nano Letters, 2014, 14, 2196-2200.
Auvinen, H.; Zhang, H.; Nonappa; Kopilow, A.; Niemelä, E. H.; Nummelin, S.; Ikkala, O.; Santos, H. A.; Linko, V.; Kostiainen, M. A. Protein Coating of DNA Nanostructures for Enhanced Stability and Immunocompatibility, Advanced Healthcare Materials, 2017, 6, 1700692.
Shaukat, A.; Anaya-Plaza, E.; Julin, S.; Linko, V.; Torres, T.; de la Escosura, A.; Kostiainen, M. A. Phthalocyanine-DNA Origami Complexes with Enhanced Stability and Optical Properties, Chemical Communications, 2020, 56, 7341-7344.
Bio:
Mauri A. Kostiainen obtained his Ph.D. in engineering physics from Helsinki University of Technology, Finland (2008). After receiving his doctoral degree, he spent 2.5 years at the Radboud University Nijmegen (The Netherlands) developing new approaches for chemical and physical virology. He returned to Aalto University in 2011 as an Academy of Finland postdoctoral fellow and joined the School of Chemical Engineering in 2013, where he is currently an Associate Professor. His research interests focus on the integration of biological and synthetic building blocks in a designed manner to create biohybrid materials.
Zoom link (with registration) for attending remotely: https://go.epfl.ch/EPFLBioETalks
IMPORTANT NOTICE: due to restrictions resulting from the ongoing Covid-19 situation, this seminar can be followed via Zoom web-streaming only, following prior one-time registration through the link above.
(note that this talk is number one of a double-feature seminar - see details of the second talk here)
Abstract:
Fully addressable DNA nanostructures, especially DNA origami, possess huge potential to serve as inherently biocompatible and versatile molecular platforms. However, their use as delivery vehicles in therapeutics is compromised by their low stability and poor transfection rates. Our studies have shown that DNA origamis can be coated non-covalently by various materials to tackle the aforementioned issues. In detail, we have demonstrated that coatings utilizing either virus capsid proteins, serum albumin, phthalocyanine dyes or cationic lipids can enhance the delivery, and especially stability of the structures. The electrostatic complexation strategy is highly modular and suitable for a wide range of DNA-based templates.
References:
Mikkilä, J.; Eskelinen, A.-P.; Niemelä, E.; Linko, V.; Frilander, M.; Törmä, P.; Kostiainen, M. A. Virus Encapsulated DNA Origami Nanostructures for Cellular Delivery, Nano Letters, 2014, 14, 2196-2200.
Auvinen, H.; Zhang, H.; Nonappa; Kopilow, A.; Niemelä, E. H.; Nummelin, S.; Ikkala, O.; Santos, H. A.; Linko, V.; Kostiainen, M. A. Protein Coating of DNA Nanostructures for Enhanced Stability and Immunocompatibility, Advanced Healthcare Materials, 2017, 6, 1700692.
Shaukat, A.; Anaya-Plaza, E.; Julin, S.; Linko, V.; Torres, T.; de la Escosura, A.; Kostiainen, M. A. Phthalocyanine-DNA Origami Complexes with Enhanced Stability and Optical Properties, Chemical Communications, 2020, 56, 7341-7344.
Bio:
Mauri A. Kostiainen obtained his Ph.D. in engineering physics from Helsinki University of Technology, Finland (2008). After receiving his doctoral degree, he spent 2.5 years at the Radboud University Nijmegen (The Netherlands) developing new approaches for chemical and physical virology. He returned to Aalto University in 2011 as an Academy of Finland postdoctoral fellow and joined the School of Chemical Engineering in 2013, where he is currently an Associate Professor. His research interests focus on the integration of biological and synthetic building blocks in a designed manner to create biohybrid materials.
Zoom link (with registration) for attending remotely: https://go.epfl.ch/EPFLBioETalks
IMPORTANT NOTICE: due to restrictions resulting from the ongoing Covid-19 situation, this seminar can be followed via Zoom web-streaming only, following prior one-time registration through the link above.
Practical information
- Informed public
- Registration required
Organizer
Contact
- Institute of Bioengineering (IBI), Dietrich REINHARD