EPFL BioE Talks SERIES "Investigating Cellular Logistics With Live-Cell STED Super-Resolution Microscopy"
Event details
| Date | 25.04.2022 |
| Hour | 16:00 › 17:00 |
| Speaker | Prof. Francesca Bottanelli, Membrane trafficking Lab, Institute for Chemistry and Biochemistry, Freie Universität Berlin (D) |
| Location | Online |
| Category | Conferences - Seminars |
| Event Language | English |
WEEKLY EPFL BIOE TALKS SERIES
Abstract:
In the lab we use gene editing and live-cell super-resolution microscopy to investigate the mechanisms of membrane homeostasis in health and disease. To be able to image physiological molecular processes in the crowded cellular cytoplasm in living cells, we developed labelling methods for stimulated emission depletion (STED) super-resolution imaging in living cells and a pipeline for the rapid generation of CRISPR-Cas9 knock ins. These methods unlocked the possibility to image dynamics at sub-50 nm spatial resolution and under near-native cellular conditions. Dynamic nanoscale microscopy of endogenously tagged machinery is revealing novel cellular roles for ARF GTPases, one of the major family of regulators of cellular membrane homeostasis. Defining the role of ARFs led to the discovery of unexplored sorting mechanisms between the endoplasmic reticulum and the Golgi apparatus. We show that bi-directional transport between the ER and the Golgi apparatus occur via a dynamic and quickly remodelling network of connecting nano-tunnels. In a similar way, Golgi-derived sorting tubules serve as a sorting station between Golgi and endo-lysosomal organelles to coordinate secretory and lysosomal trafficking via kiss and run events.
Bio:
2018 - present
Assistant Professor, Freie Universität Berlin, Institute for Chemistry and Biochemistry
2011 - 2018
Postdoctoral fellow, Yale University school of medicine, Department of Cell Biology, New Haven, CT (USA)
Advisor: James E. Rothman
2006 – 2011
PhD, Faculty of Biological Sciences, The University of Leeds (UK)
Advisor: Dr. Jürgen Denecke
2004 – 2006
MSc in Plant, Food and Environmental Biotechnology, University of Milan (IT)
2001 – 2004
BSc in Plant Biotechnology, University of Milan (IT)
Zoom link (with one-time registration for the whole series) for attending remotely: https://go.epfl.ch/EPFLBioETalks
Instructions for 1st-year Ph.D. students who are under EDBB’s mandatory seminar attendance rule:
IF you are not attending in-person in the room, please make sure to
Abstract:
In the lab we use gene editing and live-cell super-resolution microscopy to investigate the mechanisms of membrane homeostasis in health and disease. To be able to image physiological molecular processes in the crowded cellular cytoplasm in living cells, we developed labelling methods for stimulated emission depletion (STED) super-resolution imaging in living cells and a pipeline for the rapid generation of CRISPR-Cas9 knock ins. These methods unlocked the possibility to image dynamics at sub-50 nm spatial resolution and under near-native cellular conditions. Dynamic nanoscale microscopy of endogenously tagged machinery is revealing novel cellular roles for ARF GTPases, one of the major family of regulators of cellular membrane homeostasis. Defining the role of ARFs led to the discovery of unexplored sorting mechanisms between the endoplasmic reticulum and the Golgi apparatus. We show that bi-directional transport between the ER and the Golgi apparatus occur via a dynamic and quickly remodelling network of connecting nano-tunnels. In a similar way, Golgi-derived sorting tubules serve as a sorting station between Golgi and endo-lysosomal organelles to coordinate secretory and lysosomal trafficking via kiss and run events.
Bio:
2018 - present
Assistant Professor, Freie Universität Berlin, Institute for Chemistry and Biochemistry
2011 - 2018
Postdoctoral fellow, Yale University school of medicine, Department of Cell Biology, New Haven, CT (USA)
Advisor: James E. Rothman
2006 – 2011
PhD, Faculty of Biological Sciences, The University of Leeds (UK)
Advisor: Dr. Jürgen Denecke
2004 – 2006
MSc in Plant, Food and Environmental Biotechnology, University of Milan (IT)
2001 – 2004
BSc in Plant Biotechnology, University of Milan (IT)
Zoom link (with one-time registration for the whole series) for attending remotely: https://go.epfl.ch/EPFLBioETalks
Instructions for 1st-year Ph.D. students who are under EDBB’s mandatory seminar attendance rule:
IF you are not attending in-person in the room, please make sure to
- send D. Reinhard a note before noon on seminar day, informing that you plan to attend the talk online, and
- be signed in on Zoom with a recognizable user name (not a pseudonym making it difficult or impossible to be identified).
Practical information
- Informed public
- Registration required
Organizer
- Prof. Giovanni D'Angelo, EPFL
Contact
- Institute of Bioengineering (IBI), Dietrich REINHARD