EPFL BioE Talks SERIES "Mapping Precise Ramifications of Substoichiometric Electrophile-Driven Cell Signaling"
Event details
| Date | 10.05.2021 |
| Hour | 16:30 › 17:00 |
| Speaker | Prof. Yimon Aye, Laboratory of Electrophiles And Genome Operation (LEAGO), Institute of Chemical Sciences and Engineering, EPFL, Lausanne (CH) |
| Location | Online |
| Category | Conferences - Seminars |
WEEKLY EPFL BIOE TALKS SERIES
(note that this talk is number two of a double-feature seminar - see details of the first talk here)
Abstract:
Precisely timed and spatially regulated electrophilic chemical signals are slowly being implicated as bona fide signaling events in numerous cells. However, modeling these low-stoichiometry signaling events and defining the precise biological impacts of localized signals under physiologic conditions has proven to be highly challenging. This seminar will spotlight a unique set of proximity-directed chemical biology tools that enables interrogation into functional consequences of specific redox-linked events: namely, T-REX™ precision electrophile delivery and G-REX™ electrophile-ligandability profiling in living systems, and how using these technologies have enabled us to identify bona fide “first responders” that interact with native signaling electrophiles under close to endogenous redox signaling conditions (i.e., “kcat/Km”-like). Our data show that these first responders lie at nexuses between electrophile- and canonical-signaling pathways. Thus, these proteins translate information encoded by electrophiles to phosphate or ubiquitin to reroute signaling pathway flux, even at the organismal level. I will also highlight how our new ability to discover and functionally decipher precision electrophile signaling mechanisms is leveraged toward novel ligand/target discovery.
Bio:
Undergraduate Education (2000 – 2004): University of Oxford, UK
Graduate Education (2004 – 2009): Harvard University, USA
Postdoctoral Training (2009 – 2012): MIT, USA
Yimon Aye was born and raised in Burma. She moved to the UK to study for sixth form (high school) and then read chemistry at Oxford University, UK. She moved to Harvard University, USA, achieving a Ph.D. in organic chemistry under the supervision of Prof. David A. Evans. She then moved to Massachusetts Institute of Technology (USA) to research the cellular and biochemical regulatory mechanisms of the enzyme ribonucleotide reductase with Prof. JoAnne Stubbe. In her independent career at Cornell University that began in mid-2012, she set out to understand the detailed mechanisms of electrophile signaling. This impetus culminated in the development of “REX” technologies (T-REX™ delivery and G-REX™ profiling). In a parallel research program distinct from redox-dependent cell signaling, she studies proteins/pathways involved in mammalian genome maintenance and nucleotide signaling, including the mechanisms of anticancer agents in clinical use. As of August 2018, she is leading the Laboratory of Electrophiles And Genome Operation (LEAGO) at the Institute of Chemical Sciences and Engineering (ISIC), EPFL (Switzerland) as a tenured associate professor.
Zoom link (with registration) for attending remotely: https://go.epfl.ch/EPFLBioETalks
IMPORTANT NOTICE: due to restrictions resulting from the ongoing Covid-19 pandemic, this seminar can be followed via Zoom web-streaming only, (following prior one-time registration through the link above).
(note that this talk is number two of a double-feature seminar - see details of the first talk here)
Abstract:
Precisely timed and spatially regulated electrophilic chemical signals are slowly being implicated as bona fide signaling events in numerous cells. However, modeling these low-stoichiometry signaling events and defining the precise biological impacts of localized signals under physiologic conditions has proven to be highly challenging. This seminar will spotlight a unique set of proximity-directed chemical biology tools that enables interrogation into functional consequences of specific redox-linked events: namely, T-REX™ precision electrophile delivery and G-REX™ electrophile-ligandability profiling in living systems, and how using these technologies have enabled us to identify bona fide “first responders” that interact with native signaling electrophiles under close to endogenous redox signaling conditions (i.e., “kcat/Km”-like). Our data show that these first responders lie at nexuses between electrophile- and canonical-signaling pathways. Thus, these proteins translate information encoded by electrophiles to phosphate or ubiquitin to reroute signaling pathway flux, even at the organismal level. I will also highlight how our new ability to discover and functionally decipher precision electrophile signaling mechanisms is leveraged toward novel ligand/target discovery.
Bio:
Undergraduate Education (2000 – 2004): University of Oxford, UK
Graduate Education (2004 – 2009): Harvard University, USA
Postdoctoral Training (2009 – 2012): MIT, USA
Yimon Aye was born and raised in Burma. She moved to the UK to study for sixth form (high school) and then read chemistry at Oxford University, UK. She moved to Harvard University, USA, achieving a Ph.D. in organic chemistry under the supervision of Prof. David A. Evans. She then moved to Massachusetts Institute of Technology (USA) to research the cellular and biochemical regulatory mechanisms of the enzyme ribonucleotide reductase with Prof. JoAnne Stubbe. In her independent career at Cornell University that began in mid-2012, she set out to understand the detailed mechanisms of electrophile signaling. This impetus culminated in the development of “REX” technologies (T-REX™ delivery and G-REX™ profiling). In a parallel research program distinct from redox-dependent cell signaling, she studies proteins/pathways involved in mammalian genome maintenance and nucleotide signaling, including the mechanisms of anticancer agents in clinical use. As of August 2018, she is leading the Laboratory of Electrophiles And Genome Operation (LEAGO) at the Institute of Chemical Sciences and Engineering (ISIC), EPFL (Switzerland) as a tenured associate professor.
Zoom link (with registration) for attending remotely: https://go.epfl.ch/EPFLBioETalks
IMPORTANT NOTICE: due to restrictions resulting from the ongoing Covid-19 pandemic, this seminar can be followed via Zoom web-streaming only, (following prior one-time registration through the link above).
Practical information
- Informed public
- Registration required
Organizer
- Prof. Li Tang, EPFL
Contact
- Institute of Bioengineering (IBI), Dietrich REINHARD