EPFL BioE Talks SERIES "Modular Hydrogels in Organoid-Based Disease Modelling"


Event details

Date 02.05.2022
Hour 16:0017:00
Speaker Dr. Eileen Gentleman, Centre for Craniofacial & Regenerative Biology, King's College London (UK)
Location Online
Category Conferences - Seminars
Event Language English

Pathological matrix remodelling plays a central role in many human diseases, but is challenging to study as in vitro models often cannot replicate the complex 3D cell-matrix interactions that drive pathologies. In this seminar, I will discuss how we built a 3D model of the human gut that allowed us to uncover an unexpected role for a rare immune cell type called ILC1 in driving gut fibrosis in patients with inflammatory bowel diseases. We used molecular dynamics simulations to design PEG hydrogels that cross-link quickly, but can still mimic the stiffness of normal intestinal tissue. We then co-cultured encapsulated human intestinal organoids with ILC1, and using a combination of atomic force microscopy and spectroscopy and multiple particle tracking micro-rheology, found that ILC1 drive intestinal matrix remodelling through a balance of MMP9-mediated matrix degradation and TGFβ1-driven fibronectin deposition. Our findings demonstrate the potential of using hydrogels in disease modelling, and open the possibility of unravelling how pathological matrix remodelling contributes to disease.

Eileen Gentleman is a Reader in Bioengineering in the Centre for Craniofacial and Regenerative Biology at King’s College London. She joined Imperial College London in 2005 as post-doctoral research associate after completing her PhD in Biomedical Engineering at Tulane University (USA). In 2011, she was awarded a Wellcome Trust Research Career Development Fellowship and established her lab at King’s with a focus on developing materials with modifiable physical and biological properties to direct cellular responses for regenerative applications and to create disease models. She has received funding awards from the Wellcome Trust, the Medical Research Council, and the Engineering and Physical Sciences Research Council (UK). In 2013 her work in regenerative medicine was recognised with a prestigious Philip Leverhulme Prize.

Zoom link (with one-time registration for the whole series) for attending remotely: https://go.epfl.ch/EPFLBioETalks

Instructions for 1st-year Ph.D. students who are under EDBB’s mandatory seminar attendance rule:
IF you are not attending in-person in the room, please make sure to
  1. send D. Reinhard a note before noon on seminar day, informing that you plan to attend the talk online, and
  2. be signed in on Zoom with a recognizable user name (not a pseudonym making it difficult or impossible to be identified).
Students attending the seminar in-person should collect a confirmation signature after the talk - please print your own signature sheet beforehand (71 kB pdf available for download here).

Practical information

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  • Registration required