EPFL BioE Talks SERIES "Nanopipette Biosensors for Single-Cell Analysis"
Event details
| Date | 29.03.2021 |
| Hour | 16:00 › 16:30 |
| Speaker | Prof. Yuri Korchev, Faculty of Medicine, Imperial College London (UK) |
| Location | Online |
| Category | Conferences - Seminars |
WEEKLY EPFL BIOE TALKS SERIES
(note that this talk is number one of a double-feature seminar - see details of the second talk here)
Abstract:
Molecular Biology has advanced we know much about the individual molecular components that make up living cells down to the level of the individual atoms. The challenge, however, is to fully understand the functional integration of these components. This requires determining how the molecular machines that make up a living cell are organized and interact together not at the atomic length scale but on a nm scale. To do this we need to develop and applying nanoscale techniques for the visualization and quantification of cell machinery in real-time and on living cells. This will lead to detailed, quantitative models of sub-cellular structures and molecular complexes under different conditions for both normal and diseased cells.
This approach ultimately requires the development of novel biophysical methods. We have recently pioneered the development of an array of new and powerful biophysical tools based on Scanning Ion Conductance Microscopy that allow quantitative measurements and non-invasive functional imaging of single protein molecules in living cells.
Scanning ion conductance microscopy and a battery of associated innovative methods are unique among current imaging techniques, not only in spatial resolution of living and functioning cells, but also in the rich combination of imaging with other functional and dynamical interrogation methods [1]. There is a significant advance to deliver nanotechnological solutions to biosensing that are affordable, integrated, fast, capable of multiplexed detection and monitoring, and crucially to offer high selectivity for the specific detection of trace levels of analyte in biological fluids. Herein, we design a new class of nanometric field-effect-transistor (FET) sensors [2] and dubbed nexFET (nanopore extended Field Effect Transistor) [3] that combine the advantages of nanopore single molecule sensing, FETs and recognition chemistry.
The nexFET biosensors, with controllable gate voltage enable higher molecular throughput, enhanced signal-to-noise and even heightened selectivity via functionalization of the nexFET with an embedded receptor. This is shown for sensitive and selective detection of an anti-insulin antibody in the presence of its IgG isotype as well as within complex mixtures such as blood serum. Self-assembled nanoporous sensors at the tip of nanopipette can be used for simultaneous SICM imaging and chemical imaging and can be combined with FET sensors.
References:
[1]. Zhang, Y. et al. (2019). High-resolution label-free 3D mapping of extracellular pH of single living cells. Nature Comm. 10, 1-9
[2] Y Zhang, et al. Spearhead Nanometric Field-Effect Transistor Sensors for Single-Cell Analysis. (2016). ACS Nano 10, 3214-3221, DOI: 10.1021/acsnano.5b05211
[3] R Ren, et al. Nanopore extended field-effect transistor for selective single-molecule biosensing. (2017) Nature Com. 8, DOI: 10.1038/s41467-017-00549-w
Bio:
Education:
1983 ・B.Sc. in Biology & Physiology, University of St Petersburg
1984 ・M.Sc. in Physiology, University of St Petersburg
1989 ・Ph.D. in Biophysics and Cytology, Russian Academy of Sciences
Professional Career:
1984 - 1991 Research Scientist, Russian Academy of Sciences, St Petersburg, Russia
1991 - 1992 Wellcome Trust Research Fellow, St George's Hospital Medical School, UK
1992 - 1995 Post-Doctoral Research Fellow, St George's Hospital Medical School, London, UK
1995 - 1997 Post-Doctoral Research Fellow, Charing Cross & Westminster Medical School, UK
1997 - 2001 Lecturer, Faculty of Medicine, Imperial College London, UK
2001 - 2003 Senior Lecturer, Faculty of Medicine, Imperial College London, UK
2003 - 2005 Reader, Faculty of Medicine, Imperial College London, UK
2005 - present Professor, Faculty of Medicine, Imperial College London, UK
Zoom link (with registration) for attending remotely: https://go.epfl.ch/EPFLBioETalks
IMPORTANT NOTICE: due to restrictions resulting from the ongoing Covid-19 pandemic, this seminar can be followed via Zoom web-streaming only, (following prior one-time registration through the link above).
(note that this talk is number one of a double-feature seminar - see details of the second talk here)
Abstract:
Molecular Biology has advanced we know much about the individual molecular components that make up living cells down to the level of the individual atoms. The challenge, however, is to fully understand the functional integration of these components. This requires determining how the molecular machines that make up a living cell are organized and interact together not at the atomic length scale but on a nm scale. To do this we need to develop and applying nanoscale techniques for the visualization and quantification of cell machinery in real-time and on living cells. This will lead to detailed, quantitative models of sub-cellular structures and molecular complexes under different conditions for both normal and diseased cells.
This approach ultimately requires the development of novel biophysical methods. We have recently pioneered the development of an array of new and powerful biophysical tools based on Scanning Ion Conductance Microscopy that allow quantitative measurements and non-invasive functional imaging of single protein molecules in living cells.
Scanning ion conductance microscopy and a battery of associated innovative methods are unique among current imaging techniques, not only in spatial resolution of living and functioning cells, but also in the rich combination of imaging with other functional and dynamical interrogation methods [1]. There is a significant advance to deliver nanotechnological solutions to biosensing that are affordable, integrated, fast, capable of multiplexed detection and monitoring, and crucially to offer high selectivity for the specific detection of trace levels of analyte in biological fluids. Herein, we design a new class of nanometric field-effect-transistor (FET) sensors [2] and dubbed nexFET (nanopore extended Field Effect Transistor) [3] that combine the advantages of nanopore single molecule sensing, FETs and recognition chemistry.
The nexFET biosensors, with controllable gate voltage enable higher molecular throughput, enhanced signal-to-noise and even heightened selectivity via functionalization of the nexFET with an embedded receptor. This is shown for sensitive and selective detection of an anti-insulin antibody in the presence of its IgG isotype as well as within complex mixtures such as blood serum. Self-assembled nanoporous sensors at the tip of nanopipette can be used for simultaneous SICM imaging and chemical imaging and can be combined with FET sensors.
References:
[1]. Zhang, Y. et al. (2019). High-resolution label-free 3D mapping of extracellular pH of single living cells. Nature Comm. 10, 1-9
[2] Y Zhang, et al. Spearhead Nanometric Field-Effect Transistor Sensors for Single-Cell Analysis. (2016). ACS Nano 10, 3214-3221, DOI: 10.1021/acsnano.5b05211
[3] R Ren, et al. Nanopore extended field-effect transistor for selective single-molecule biosensing. (2017) Nature Com. 8, DOI: 10.1038/s41467-017-00549-w
Bio:
Education:
1983 ・B.Sc. in Biology & Physiology, University of St Petersburg
1984 ・M.Sc. in Physiology, University of St Petersburg
1989 ・Ph.D. in Biophysics and Cytology, Russian Academy of Sciences
Professional Career:
1984 - 1991 Research Scientist, Russian Academy of Sciences, St Petersburg, Russia
1991 - 1992 Wellcome Trust Research Fellow, St George's Hospital Medical School, UK
1992 - 1995 Post-Doctoral Research Fellow, St George's Hospital Medical School, London, UK
1995 - 1997 Post-Doctoral Research Fellow, Charing Cross & Westminster Medical School, UK
1997 - 2001 Lecturer, Faculty of Medicine, Imperial College London, UK
2001 - 2003 Senior Lecturer, Faculty of Medicine, Imperial College London, UK
2003 - 2005 Reader, Faculty of Medicine, Imperial College London, UK
2005 - present Professor, Faculty of Medicine, Imperial College London, UK
Zoom link (with registration) for attending remotely: https://go.epfl.ch/EPFLBioETalks
IMPORTANT NOTICE: due to restrictions resulting from the ongoing Covid-19 pandemic, this seminar can be followed via Zoom web-streaming only, (following prior one-time registration through the link above).
Practical information
- Informed public
- Registration required
Organizer
- Prof. Georg Fantner, EPFL
Contact
- Institute of Bioengineering (IBI), Dietrich REINHARD