EPFL BioE Talks SERIES "Streamlining Fragment-Based Chemoproteomics for in Cell Screening of Large Electrophile Libraries"


Event details

Date 10.05.2021
Hour 16:0016:30
Speaker Prof. Steven Gygi, Department of Cell Biology, Harvard Medical School, Boston, MA (USA)
Location Online
Category Conferences - Seminars
(note that this talk is number one of a double-feature seminar - see details of the second talk here)

Understanding the cellular role of each human protein requires the development of small-molecule reagents that modulate protein interactions, expression, and function. Current methods, however, lack the throughput needed to screen large chemical libraries for interactions across the entire proteome. To facilitate this endeavor, we have redesigned the entire workflow for activity-based protein profiling (ABPP) of reactive cysteine residues with an eye towards screening large libraries. We broadly screened cysteine reactivity for a library of 285 electrophiles in duplicate using intact HCT116 cells in just 4 days. More than 20,000 cysteines were quantified with >6,000 proteins harboring reactive cysteine sites. We have repeated the screen in 3 additional lines and have started to screen a full library of 3,200 compounds in K562 cells.

Steven Gygi received his Ph.D. in Pharmacology and Toxicology from the University of Utah in 1995.  He performed postdoctoral work with Ruedi Aebersold at the University of Washington from 1996-2000 studying mass spectrometry-based proteomics.  Today, his lab at Harvard Medical School develops proteomic technologies to systematically measure protein properties including their expression levels, interactions, and modifications.  Sample multiplexing using tandem mass tags (TMT) currently allows 18 different cellular states to be simultaneously analyzed in his lab.

Zoom link (with registration) for attending remotely: https://go.epfl.ch/EPFLBioETalks

IMPORTANT NOTICE: due to restrictions resulting from the ongoing Covid-19 pandemic, this seminar can be followed via Zoom web-streaming only, (following prior one-time registration through the link above).

Practical information

  • Informed public
  • Registration required