Focusing on mitochondria with super-resolution microscopy
Event details
| Date | 11.06.2012 |
| Speaker | Prof. Stefan Jakobs, Max Planck Institute for Biophysical Chemistry |
| Location |
SV 1717A
|
| Category | Conferences - Seminars |
Mitochondria are essential organelles in all eukaryotic cells. Their dysfunction is related to numerous diseases including several devastating neurodegenerative diseases. The nanoscale distributions of proteins within mitochondria are largely unexplored, due to the small dimensions of the mitochondria and the high protein packaging densities in the mitochondrial membranes. The first part of the talk will report on our recent studies on sub-mitochondrial protein distributions and on the heterogeneity of mitochondria within and between cells using STED nanoscopy in combination with quantitative image analysis. In the second part of the talk I will discuss our recent progress in the design and application of reversibly switchable fluorescent proteins (RSFPs). RSFPs can be repeatedly photoswitched between fluorescent and non-fluorescent states by irradiation with distinct light wavelengths, a property that can be exploited to overcome the diffraction barrier in live cell imaging utilizing low light intensities. Previous RSFPs allowed only a limited number of switching cycles before photodestruction. We have recently generated novel RSFPs exhibiting strong resistance against photobleaching and switching fatigue. They are therefore well suited for reversible saturable optical (fluorescence) transition (RESOLFT) nanoscopy enabling the recording of raw data images from living cells and tissues with low levels of light.
Practical information
- General public
- Free
Organizer
- Lasser Theo <[email protected]>
Contact
- Lasser Theo <[email protected]>