Functional MRI in Mice: Towards the Analysis of Networks
Event details
| Date | 01.04.2014 |
| Hour | 16:15 |
| Speaker | Prof. Markus Rudin, University of Zurich and ETH Zurich (CH) |
| Location | |
| Category | Conferences - Seminars |
BIOENGINEERING SEMINAR
Abstract:
Functional magnetic resonance imaging (fMRI) in rodents is attractive in many regards: 1. By combining fMRI with established invasive readouts of neuronal function mechanistic information on the link of the hemodynamic response to the underlying neural activity can be obtained. 2. Genetically engineered mouse lines allow identifying the specific molecular mediators involved in signal processing. 3. The relatively simple morphology of the cortical vasculature enables detailed analyses of the functional topology and its rearrangements following focal CNS injury. 4. Modern fMRI techniques allow for full three-dimensional coverage of the brain essential for the elucidation of large scale networks involved in specific tasks, during pharmacological activation or at rest.
Challenges in mouse fMRI are linked to the small dimensions and the high demands on spatial resolution, to animal physiology, which should be stable enough to allow for detection of percent changes in signal intensity, and to potential interference by anaesthesia. Yet, technical solutions are available and mouse fMRI is becoming established. The different aspects of rodent fMRI will be illustrated. Mechanistic information on neurovascular coupling was obtained by combined fMRI/fiber-optics measurement of the bulk Ca2+ signal in the rat using a fluorescent ligand; a potential role of glia in determining the vascular signal has been implicated. A critical aspect in rodent fMRI is the impact of anesthesia. We have therefore compared four anesthetics with regard to their effects on stimulus evoked fMRI responses and functional connectivity patterns. Anesthetics affect signals in a differential manner that can be rationalized in terms of anesthesia depth, mechanism of action and effects on the cerebral vasculature. Resting-state fMRI has been applied to analyse alteration in network patterns in a murine model of cerebral amyloidosis. Finally fMRI following the administration of serotonin agonists in mice have revealed activation in brain structures associated with serotonergic signaling, which was found altered in a mouse model of acquired depressive behavior. Interestingly, changes in serotonergic networks were transmitted to the offsprings of affected generation.
Bio:
Markus Rudin is full professor for Molecular Imaging and Functional Pharmacology at the Institute for Biomedical Engineering (D-ITET) since June 2006. Since March 2005 he is also full professor for Molecular Imaging and Functional Pharmacology at the University of Zürich both at the Institute for Biomedical Engineering and the Institute for Pharmacology and Toxicology.
Markus Rudin (born 1953, from Lauwil BL) received his diploma in chemistry at the ETHZ in 1976 and his PhD at the Laboratory for Physical Chemistry in 1981 in the field of electron spin resonance / electron-nuclear double resonance, followed by a post-doctorate in the same area. In 1983 he moved to biomedical imaging, joining Sandoz AG to build up a biomedical imaging group initially focused on magnetic resonance imaging. He received his basic training in imaging at the Biocenter of the University of Basel. Within Sandoz AG, later Novartis Pharma AG, he became head of the Biophysics Group, head of the In-vivo Models Unit and finally head of the Analytical and Imaging Science Unit within Discovery Technologies at the Novartis Institutes for Biomedical Research. In this function he was also deputy head of Discovery Technologies until 2005. In 1997 he became Privat Dozent for Biophysics at the University of Basel. Since March 2005, he is member of the Research Council of the Swiss National Science Foundation.
Markus Rudin is heading a research group at the animal imaging center of UZH and ETH located at ETH Hönggerberg focusing on MRI and optical imaging methods (fluorescence tomography). His research focus is the development of non-invasive imaging techniques for studying structure, physiology, and metabolism of tissue as well as cellular and molecular events in the intact organism, in particular assays for monitoring signal transduction pathways. Biomedical applications are in neuroscience and metabolic diseases.
Speaker's personal web page
Abstract:
Functional magnetic resonance imaging (fMRI) in rodents is attractive in many regards: 1. By combining fMRI with established invasive readouts of neuronal function mechanistic information on the link of the hemodynamic response to the underlying neural activity can be obtained. 2. Genetically engineered mouse lines allow identifying the specific molecular mediators involved in signal processing. 3. The relatively simple morphology of the cortical vasculature enables detailed analyses of the functional topology and its rearrangements following focal CNS injury. 4. Modern fMRI techniques allow for full three-dimensional coverage of the brain essential for the elucidation of large scale networks involved in specific tasks, during pharmacological activation or at rest.
Challenges in mouse fMRI are linked to the small dimensions and the high demands on spatial resolution, to animal physiology, which should be stable enough to allow for detection of percent changes in signal intensity, and to potential interference by anaesthesia. Yet, technical solutions are available and mouse fMRI is becoming established. The different aspects of rodent fMRI will be illustrated. Mechanistic information on neurovascular coupling was obtained by combined fMRI/fiber-optics measurement of the bulk Ca2+ signal in the rat using a fluorescent ligand; a potential role of glia in determining the vascular signal has been implicated. A critical aspect in rodent fMRI is the impact of anesthesia. We have therefore compared four anesthetics with regard to their effects on stimulus evoked fMRI responses and functional connectivity patterns. Anesthetics affect signals in a differential manner that can be rationalized in terms of anesthesia depth, mechanism of action and effects on the cerebral vasculature. Resting-state fMRI has been applied to analyse alteration in network patterns in a murine model of cerebral amyloidosis. Finally fMRI following the administration of serotonin agonists in mice have revealed activation in brain structures associated with serotonergic signaling, which was found altered in a mouse model of acquired depressive behavior. Interestingly, changes in serotonergic networks were transmitted to the offsprings of affected generation.
Bio:
Markus Rudin is full professor for Molecular Imaging and Functional Pharmacology at the Institute for Biomedical Engineering (D-ITET) since June 2006. Since March 2005 he is also full professor for Molecular Imaging and Functional Pharmacology at the University of Zürich both at the Institute for Biomedical Engineering and the Institute for Pharmacology and Toxicology.
Markus Rudin (born 1953, from Lauwil BL) received his diploma in chemistry at the ETHZ in 1976 and his PhD at the Laboratory for Physical Chemistry in 1981 in the field of electron spin resonance / electron-nuclear double resonance, followed by a post-doctorate in the same area. In 1983 he moved to biomedical imaging, joining Sandoz AG to build up a biomedical imaging group initially focused on magnetic resonance imaging. He received his basic training in imaging at the Biocenter of the University of Basel. Within Sandoz AG, later Novartis Pharma AG, he became head of the Biophysics Group, head of the In-vivo Models Unit and finally head of the Analytical and Imaging Science Unit within Discovery Technologies at the Novartis Institutes for Biomedical Research. In this function he was also deputy head of Discovery Technologies until 2005. In 1997 he became Privat Dozent for Biophysics at the University of Basel. Since March 2005, he is member of the Research Council of the Swiss National Science Foundation.
Markus Rudin is heading a research group at the animal imaging center of UZH and ETH located at ETH Hönggerberg focusing on MRI and optical imaging methods (fluorescence tomography). His research focus is the development of non-invasive imaging techniques for studying structure, physiology, and metabolism of tissue as well as cellular and molecular events in the intact organism, in particular assays for monitoring signal transduction pathways. Biomedical applications are in neuroscience and metabolic diseases.
Speaker's personal web page
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