Genome Architecture Mapping (GAM): an Orthogonal Approach to Map Chromatin Contacts Genome-Wide
Event details
| Date | 16.01.2017 |
| Hour | 11:00 |
| Speaker | Prof. Ana Pombo, Humboldt University, Berlin (D) |
| Location | |
| Category | Conferences - Seminars |
BIOENGINEERING SEMINAR
Abstract:
The organization of the genome in the nucleus and the interactions of genes with their regulatory elements are key features of transcriptional control and their disruption causes disease. Technologies based on chromosome conformation capture (3C) have profoundly expanded our understanding of the role of genome architecture in gene regulation. We introduce Genome Architecture Mapping (GAM), an orthogonal genome-wide method for measuring three-dimensional chromatin topology, which requires small cell numbers and can be applied directly in tissue. Exploration of the most prominent chromatin contacts detected using GAM in the nucleus of mouse ES cells identifies the most specific chromatin contacts between active genes and enhancers across very large genomic distances. Our results highlight a previously inaccessible complexity in genome architecture and a major role for contacts related with gene expression in the structural organisation of the genome in mammalian nuclei.
Bio:
Originally from Lisbon, Portugal
BSc: University of Lisbon, Lisbon, Portugal
DPhil: University of Oxford, UK
Abstract:
The organization of the genome in the nucleus and the interactions of genes with their regulatory elements are key features of transcriptional control and their disruption causes disease. Technologies based on chromosome conformation capture (3C) have profoundly expanded our understanding of the role of genome architecture in gene regulation. We introduce Genome Architecture Mapping (GAM), an orthogonal genome-wide method for measuring three-dimensional chromatin topology, which requires small cell numbers and can be applied directly in tissue. Exploration of the most prominent chromatin contacts detected using GAM in the nucleus of mouse ES cells identifies the most specific chromatin contacts between active genes and enhancers across very large genomic distances. Our results highlight a previously inaccessible complexity in genome architecture and a major role for contacts related with gene expression in the structural organisation of the genome in mammalian nuclei.
Bio:
Originally from Lisbon, Portugal
BSc: University of Lisbon, Lisbon, Portugal
DPhil: University of Oxford, UK
Practical information
- Informed public
- Free