IMX Talks - Micro electro-mechanical physiological systems (MEMPhyS)

Microphysiological systems (MPS) aim to recapitulate in vitro relevant (patho)physiology and functionality of tissues and (parts of) organs [1]. For this purpose, MPS need to accommodate 3D cell constructs and provide a comprehensive microenvironment that promotes cells’ target phenotype and functionality [2]. The microenvironment must include realistic cues, such as mechanical stress, chemical gradients and electrical stimulation among others. Moreover, monitoring continuously the composition of the microenvironment and the health of the tissues is central to the assessment of the relevance and reproducibility of the model [3]. Continuous monitoring entails the integration of sensors within MPS which quantify important, tissue-specific parameters such as e.g. flow rate of perfused fluid, presence and concentration of analytes, electrical impedance of tissues, contractile kinetics, electrogenic activity. Our research at TU Delft correspondingly aims to develop MPS capable of electro-mechanical (EM) stimulation and sensing of cell constructs and tissues, thereby properly turning MPS into actual MEMPhyS and hence into a subset of bioelectronic devices dual to implantables.
In this presentation I will summarize the rationale behind MEMPhyS, and exemplify the potential of the electro-mechanical approach to MPS with several examples from our works, including a technology platform for engineered heart tissues [4], FET-based charge sensors [5], a microfluidic device with integrated electrodes for tissue barrier assessment across a microporous membrane [6], and 3D microelectrode arrays [7]. I will conclude outlining the perspectives opened by our work and its embedding within current projects and international standardization initiatives fostering wider adoption of MPS technology.