Mapping regulatory genetic interaction in membrane trafficking
Event details
| Date | 12.02.2015 |
| Hour | 13:15 › 14:15 |
| Speaker |
Dr. Prisca Liberali Institute of Molecular Life Sciences, University of Zürich |
| Location | |
| Category | Conferences - Seminars |
Endocytosis is critical for cellular physiology and thus highly regulated. To identify regulatory interactions controlling the endocytic membrane system, we conducted 13 RNAi screens on multiple endocytic activities and their downstream organelles. Combined with image analysis of thousands of single cells per perturbation and their cell-to-cell variability, this created a high-quality and cross-comparable quantitative dataset. Unbiased analysis revealed novel emergent properties of the endocytic membrane system and how its complexity evolved, and distinct programs of regulatory control that co-regulate specific subsets of endocytic uptake routes and organelle abundances. We show that these subset effects allow the mapping of functional regulatory interactions and their interaction motifs between kinases, membrane trafficking machinery, and the cytoskeleton at a large scale, some of which we further characterise. This work presents a powerful approach to identify regulatory interactions in complex cellular systems from parallel single-gene or double-gene perturbation screens in human cells and yeast. Unpublished data on the molecular basis of the cell-to-cell variability determined by the cell cycle and the microenvironment will also be presented.
Practical information
- Informed public
- Free
- This event is internal
Organizer
- Prof. Gisou van der Goot
Contact
- Geneviève Rossier, x31792