Multi-Micron Crisscross Structures Grown from DNA-Origami Slats


Event details

Date 22.09.2023
Hour 12:1513:15
Speaker Prof. William M. Shih, Harvard Medical School, Dana-Farber Cancer Institute and Wyss Institute for Biologically Inspired Engineering, Boston, MA (USA)
Location Online
Category Conferences - Seminars
Event Language English
Living systems achieve robust self-assembly across a wide range of length scales. In the synthetic realm, nanofabrication strategies such as DNA origami have enabled robust self-assembly of submicron-scale shapes from a multitude of single-stranded components. To achieve greater complexity, subsequent hierarchical joining of origami can be pursued. However, erroneous and missing linkages restrict the number of unique origami that can be practically combined into a single design. We have extended crisscross polymerization, a strategy previously demonstrated with single-stranded components, to DNA-origami “slats” for fabrication of custom multi-micron shapes with user-defined nanoscale surface patterning. Using a library of ~2000 strands that are combinatorially arranged to create unique DNA-origami slats, we have realized finite structures composed of >1000 uniquely addressable slats, with a mass exceeding five gigadaltons and with lateral dimensions of roughly two microns, as well as a multitude of periodic structures. Robust production of target crisscross structures is enabled through strict control over initiation, rapid growth and minimal premature termination, and highly orthogonal binding specificities. Thus crisscross growth provides a route for prototyping and scalable production of structures integrating thousands of unique components (i.e. origami slats) that each are sophisticated and molecularly precise.

William Shih is a Professor in the Department of Biological Chemistry and Molecular Pharmacology at Harvard Medical School and the Department of Cancer Biology at the Dana-Farber Cancer Institute and a Founding Core Faculty member at the Wyss Institute for Biologically Inspired Engineering at Harvard. He studied Biochemical Sciences at Harvard for his A.B. and Biochemistry at Stanford for his Ph.D. He completed a postdoctoral fellowship at The Scripps Research Institute and has since been back at Harvard as a faculty member. Recognitions received include NIH Director’s New Innovator Award, Blavatnik National Award Finalist in the Physical Sciences, Foresight Prize Award in Experimental Nanotechnology, and Rozenberg Tulip Award in DNA Computing.

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