New frontiers in super-resolution biological imaging and optogenetic probing of neuronal circuits
Event details
| Date | 05.05.2010 |
| Hour | 13:30 |
| Speaker | Alipasha Vaziri - Janelia Farm Research Campus, Howard Hughes Medical Institute |
| Location |
SV1717a
|
| Category | Conferences - Seminars |
Recent advances in optical microscopy have enabled imaging of biological samples beyond the diffraction limit at nanometer resolution. A common shortcoming has been that the imaging depth was limited to a fraction of an optical wavelength. Yet, to study whole cells, the extension of these methods to a 3D-super-resolution technique at depth is required. I will present how we have overcome this limitation by utilizing the two-photon excitation technique of temporal focusing in which the spectral properties of ultrafast optical pulses are used to control the axial intensity distribution in space. Using this technique we have demonstrated multilayer super-resolution imaging at ~50nm lateral resolution over an axial range of ~10μm in various biological samples.
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Practical information
- General public
- Free