Probing Biological Dynamics in the Native State: From Long-Term Brain Electrophysiology to Subcellular Dynamic Imaging


Event details

Date and time 02.03.2021 16:0017:00  
Speaker Dr Tian-Ming Fu
Category Conferences - Seminars
Please note that the schedule of the event still has to be confirmed

The urgency to probe and understand dynamics in biology is impeded by a major challenge in bioinstrumentation development. The large dynamic range of biological processes—interactions of molecules within milliseconds result in changes across whole-organisms over years—calls for instruments with both high spatiotemporal resolution and large-scale long-term coverage. However, high resolution measurement often requires frequent and invasive sampling, which limits the spatiotemporal coverage of the instruments. In this seminar, I will present two independent yet complementary approaches that tackle this challenge. First, I will introduce a new paradigm—syringe-injectable mesh electronics—for seamlessly merging electronics with mammalian brains. The gliosis-free and three-dimensional interpenetrated brain-electronics interface enables stable stimulation and recording from the same neurons and neural circuits over a year. I will then discuss the application of mesh electronics to retina electrophysiology in awake mice. Second, I will describe a novel multimodal optical scope with adaptive imaging correction (MOSAIC) to observe subcellular dynamics inside multicellular organisms with high spatiotemporal resolution, large imaging depth and low phototoxicity. I will present applications of MOSAIC to various model organisms, including transcription factor kinetics in embryoid bodies, axonal targeting in Drosophila, cancer metastasis and embryogenesis in Caenorhabditis elegans and zebrafish. Both the electrical and optical approaches opened up new windows to probe dynamics in biology with minimum perturbation and expanded spatiotemporal ranges.
Biography: Dr. Tian-Ming Fu obtained his B.S. in Math and Physics from Tsinghua University in 2011. He did his Ph.D. with Dr. Charles Lieber at Harvard University. His graduate work focused on the development of syringe-injectable mesh electronics ( for stable long-term in vivo brain electrophysiology. The mesh electronics open up new windows to track single neuron changes in visual perception, learning and memory in rodent and nonhuman primate. Upon receiving his Ph.D., he joined Dr. Eric Betzig’s lab at Howard Hughes Medical Institute Janelia Research Campus as a postdoc. Dr. Fu developed a multimodal optical scope with adaptive imaging correction (MOSAIC, for 4D high resolution imaging of subcellular dynamics multicellular organisms, including Caenorhabditis elegans, Drosophila, and zebrafish. Dr. Fu has received recognitions including the Smith Family Graduate Science and Engineering Fellowship and Materials Research Society (MRS) Graduate Student Award. His work has been selected as Top 10 World Changing Ideas by Scientific American and Most Notable Research Advances of the Year by Chemical & Engineering News.