Rational protein purification from transgenic feedstock – tobacco leaf extract as a challenging example
Event details
| Date | 02.12.2014 |
| Hour | 09:15 › 10:15 |
| Speaker | Dr. Johannes F. Buyel, M. Sc., Institute for Molecular Biotechnology, RWTH Aachen University |
| Location | |
| Category | Conferences - Seminars |
Many feed streams in the biopharmaceutical production of proteins, e.g. monoclonal antibodies, have to be clarified before the dissolved target can be isolated, e.g. by chromatography. Disposable filters, flocculants and precipitation methods offer a cost-effective, fast and safe way to achieve this goal. After clarification, chromatographic unit operations are most frequently used to purify the target protein until the required purity is reached, typically >99% for pharmaceutical applications. The seminar will highlight some recent achievements in clarifying biological feedstocks derived from plants and then focus on the rational design and optimization of downstream purification strategies for recombinant target proteins. Topics such as modeling chromatographic separations and planning experimental verification will be covered.
Practical information
- Informed public
- Free
Organizer
- Prof. F. Wurm, LBTC
Contact
- Lucia Baldi