Resequencing DNA microarray: A powerful tool for identification of known and novel emerging pathogens?
Event details
| Date | 16.02.2009 |
| Hour | 11:00 |
| Speaker | Dr Nicolas Berthet (Institut Pasteur) |
| Location | |
| Category | Conferences - Seminars |
The development of DNA microarray as diagnostic tool for identification of pathogens offers interesting perspectives. Indeed, this technology allows unbiased and simultaneous identification of a huge number of bacterial and viral agents. In this case, the use of resequencing DNA microarray allows to obtain more precise identification of pathogens and to enlarge the detection spectrum in comparison with classical microarray. Until nowadays, this resequencing microarray was poorly exploited for diagnostic of infectious disease, particularly in virology. The aim of our work consisted to develop and validate resequencing microarray for identification of a large series of bacterial and viral pathogens originating from diverse biological samples. Based on the Affymetrix technology, two successive generations of microarrays were developed for detection of both bacterial agents, associated to their pathogenicity genes and their conferring antibiotic resistances, and viral agents. Our results demonstrated the interest of this technology for pathogens identification in different clinical situations. Moreover, discrimination capacity of this microarray was showed for the detection of close variants or for identification of novel variants attached to a viral family. All together, our results confirmed advantages of this technology in the virology field and open novel routes of optimisation for the development of a third generation of microarray with enlarged spectrum of viral detection.
Practical information
- General public
- Free
Contact
- Prof Stewart Cole