Sensing Single Biomolecules using Protein and Solid-State Nanopores
Event details
| Date | 13.03.2014 |
| Hour | 10:15 |
| Speaker |
Prof. Amit Meller, The Technion – Israel Institute of Technology, Haifa (IL) and Boston University, Boston, MA (USA) Bio: 2010- Professor of Biomedical Engineering, Technion - Israel Institute of Technology, Israel 2010- World-Class University visiting Professor, Department of Biophysics and Chemical Biology, Seoul National University, Seoul, S. Korea 2006- Associate Professor of Biomedical Engineering, Associate Professor of Physics, Boston University 2000 - 2006 Rowland Senior Fellow, PI of the Single Molecule Biophysics lab, Rowland Institute, Harvard University 1998 - 2000 Postdoc Fellow, Department of Molecular and Cellular Biology, Harvard University with Prof. Dan Branton 1997 - 1998 Postdoc Fellow, Department of Molecular Genetics, Weizmann Institute of Science with Prof. Doron Lancet 1997 Ph.D. Physics, Weizmann Institute of Science 1993 M.Sc. Physics, Weizmann Institute of Science 1989 B.Sc. Physics and Astronomy, Tel Aviv University |
| Location | |
| Category | Conferences - Seminars |
BIOENGINEERING SEMINAR
Abstract:
Nanopores can be used to detect and characterize unlabeled biomolecules, and widely believed to be a main future platform for direct, single molecule sequencing of DNA, RNA and perhaps proteins.(1) Controlling and tuning the capture rate and the translocation speed of biomolecules through nanopores remains to be a main challenge for this technology, limiting their wider application. In this lecture I will discuss two physical methods to: (i) enhance the capture rate of DNA molecules into solid-state using salt gradients thus enabling ultra sensitive sensing down to attomoles (2), and (ii) slowing down the translocation speed of DNA and proteins through nanopores using a novel optoelectronic effect that can be switched on/off in a fraction of a millisecond.(3)
Nanpores can also be used to detect and map DNA and RNA-protein interactions. I will discuss single molecule nanopore measurements of Poly Adenine Binding Proteins (PABPs) associated with translation regulation with poly-Adenine RNAs(4), as well as the interactions of transcription factors with genomic DNA
(1). Wanunu, M. & Meller, A. in Laboratory Manual on Single Molecules Vol. 395-420 (eds T. Ha & P. Selvin) (Cold Spring Harbor Press, 2008).
(2). Wanunu, M., Morrison, W., Rabin, Y., Grosberg, A. Y. & Meller, A. Electrostatic Focusing of Unlabeled DNA into Nanoscale Pores using a Salt Gradient. Nature Nanotechnology 5, 160-165 (2010).
(3). Di Fiori, N., Squires, A., Bar, D., Gilboa, T., Moustakas, T. and A. Meller. Optoelectronic control of surface charge and translocation dynamics in solid-state nanopores. Nature Nanotechnology 8, 946–951 (2013).
(4). Lin, J., Fabian, M., Sonenberg, N. and A. Meller, Nanopore Detachment Kinetics of Poly(A) Binding Proteins from RNA Molecules Reveals the Critical Role of C-Terminus Interactions. Biophysical Journal 102, 1427–1434 (2012).
Abstract:
Nanopores can be used to detect and characterize unlabeled biomolecules, and widely believed to be a main future platform for direct, single molecule sequencing of DNA, RNA and perhaps proteins.(1) Controlling and tuning the capture rate and the translocation speed of biomolecules through nanopores remains to be a main challenge for this technology, limiting their wider application. In this lecture I will discuss two physical methods to: (i) enhance the capture rate of DNA molecules into solid-state using salt gradients thus enabling ultra sensitive sensing down to attomoles (2), and (ii) slowing down the translocation speed of DNA and proteins through nanopores using a novel optoelectronic effect that can be switched on/off in a fraction of a millisecond.(3)
Nanpores can also be used to detect and map DNA and RNA-protein interactions. I will discuss single molecule nanopore measurements of Poly Adenine Binding Proteins (PABPs) associated with translation regulation with poly-Adenine RNAs(4), as well as the interactions of transcription factors with genomic DNA
(1). Wanunu, M. & Meller, A. in Laboratory Manual on Single Molecules Vol. 395-420 (eds T. Ha & P. Selvin) (Cold Spring Harbor Press, 2008).
(2). Wanunu, M., Morrison, W., Rabin, Y., Grosberg, A. Y. & Meller, A. Electrostatic Focusing of Unlabeled DNA into Nanoscale Pores using a Salt Gradient. Nature Nanotechnology 5, 160-165 (2010).
(3). Di Fiori, N., Squires, A., Bar, D., Gilboa, T., Moustakas, T. and A. Meller. Optoelectronic control of surface charge and translocation dynamics in solid-state nanopores. Nature Nanotechnology 8, 946–951 (2013).
(4). Lin, J., Fabian, M., Sonenberg, N. and A. Meller, Nanopore Detachment Kinetics of Poly(A) Binding Proteins from RNA Molecules Reveals the Critical Role of C-Terminus Interactions. Biophysical Journal 102, 1427–1434 (2012).
Links
Practical information
- Informed public
- Free
Organizer
- Prof. Aleksandra Radenovic