"Signal Transduction during Axon Guidance Investigated with Image Cross-Correlation Spectroscopy and FRET"
Event details
| Date | 17.12.2010 |
| Hour | 12:15 |
| Speaker | Benjamin Rappaz, McGill University, Montréal, Canada |
| Location |
AI1153
|
| Category | Conferences - Seminars |
During central nervous system development neurons extend axons over relatively great distance to reach their ultimate synaptic targets. Netrin is one of the main axon guidance cues whose effect is mediated by its two receptors Unc5 and DCC. DCC alone is known to promote attraction, whereas DCC in combination with Unc5 promotes repulsion.
My current project proposes to investigate the molecular interactions between Netrin and its receptors, and part of the downstream signaling mechanisms involved using state of the art optical imaging techniques in living neurons.
Image Cross-Correlation Spectroscopy (ICCS) and Fluorescence Resonance Energy Transfer (FRET) are two complementary imaging techniques which measure co-localization of fluorescently tagged proteins.
In this presentation, I will introduce the ICCS theory and present Unc5/DCC colocalisation data I obtained with this technique. FRET was then used to measure the spatio-temporal activation of Cdc42, a small Rho GTPase activated downstream of DCC which promotes filipodia growth.
Practical information
- General public
- Free