Single-Particle Tracking of Small Nanoparticles in Nanocapillaries
Event details
| Date | 03.09.2018 |
| Hour | 15:15 › 16:15 |
| Speaker | Prof. Sanli Faez, Debye Institute for Nanomaterials Research, Utrecht University, Utrecht (NL) |
| Location | |
| Category | Conferences - Seminars |
BIOENGINEERING SEMINAR
Abstract:
I introduce nanoCapillary Electrokinetic Tracking (nanoCET), an optofluidic platform for continuously measuring the electrophoretic mobility of a single colloidal nanoparticles or macromolecule in vitro with millisecond time resolution and high charge sensitivity. This platform uses a nanobore optical fiber in which liquids flow inside the light-guiding core and nanoparticles are tracked using elastic light scattering. Our group has achieved experimental tracking of nanoparticles down to 10 nm in size inside an aqueous environment. I will explain the necessary steps for extending this method to probing single biomolecules.
To further extend the sensing capability of nanoCET to dense biological samples, transport of particles into and through the nanobore fiber should be controlled over long measurement periods. At this sizes pumping is only possible by electroosmosis, but also involve major challenges. I will discuss the mechanism of jamming at the channel entry and how to avoid it, which is an important step for using nanobore fibers and other nanopore structures.
Realizing such a flow control in the nanoCET platform will immensely facilitate the daunting challenge of monitoring biochemical or catalytic reactions on a single entity over a wide range of timescales. The unique measurement capabilities of this platform are relevant for a wide range of applications in colloid science, analytical biochemistry, and medical diagnostics.
Bio:
(from https://sanlifaez.github.io/about/)
I am an assistant professor at the Physics department of Utrecht University. I am also a member of Utrecht Young Academy and taskforce open-science.
I studied Physics at the Sharif University of Technology and recieved my master’s degree in Nanotechnology from University of Twente. My master and PhD projects were both on wave propagation in random media and Anderson localization, at the (former) FOM institute AMOLF, under the supervision of Ad Lagendijk. I recieved my doctorate degree in 2011 from the Univesity of Amsterdam. I joined the Sandoghdar division at the Max Planck Institute for the Science of Light as a post-doc, where I devised a new scheme for coherent coupling between single organic molecules and dielectric waveguides. In August 2013, I moved to the Single-Molecule Optics group, led by Michel Orrit, at the Leiden institute of Physics and started working on tracing single electrons using single molecule spectroscopy. During my stay at Leiden, I also developed the nanocapillary electrophoretic tracking (nanoCET) technique with the goal of studying rapid changes in the electrophoretic mobility of single mobile nanoparticles.
In August 2015, together with Allard Mosk, we started a new research group named Physics of Light in Complex Systems,nanoLINX. In this group, I am the principle investigator for the research direction nanoElectroPhotonics.
My hobbies are rowing, listening to and making podcasts, and making Physics-related table-top demonstrations.
Abstract:
I introduce nanoCapillary Electrokinetic Tracking (nanoCET), an optofluidic platform for continuously measuring the electrophoretic mobility of a single colloidal nanoparticles or macromolecule in vitro with millisecond time resolution and high charge sensitivity. This platform uses a nanobore optical fiber in which liquids flow inside the light-guiding core and nanoparticles are tracked using elastic light scattering. Our group has achieved experimental tracking of nanoparticles down to 10 nm in size inside an aqueous environment. I will explain the necessary steps for extending this method to probing single biomolecules.
To further extend the sensing capability of nanoCET to dense biological samples, transport of particles into and through the nanobore fiber should be controlled over long measurement periods. At this sizes pumping is only possible by electroosmosis, but also involve major challenges. I will discuss the mechanism of jamming at the channel entry and how to avoid it, which is an important step for using nanobore fibers and other nanopore structures.
Realizing such a flow control in the nanoCET platform will immensely facilitate the daunting challenge of monitoring biochemical or catalytic reactions on a single entity over a wide range of timescales. The unique measurement capabilities of this platform are relevant for a wide range of applications in colloid science, analytical biochemistry, and medical diagnostics.
Bio:
(from https://sanlifaez.github.io/about/)
I am an assistant professor at the Physics department of Utrecht University. I am also a member of Utrecht Young Academy and taskforce open-science.
I studied Physics at the Sharif University of Technology and recieved my master’s degree in Nanotechnology from University of Twente. My master and PhD projects were both on wave propagation in random media and Anderson localization, at the (former) FOM institute AMOLF, under the supervision of Ad Lagendijk. I recieved my doctorate degree in 2011 from the Univesity of Amsterdam. I joined the Sandoghdar division at the Max Planck Institute for the Science of Light as a post-doc, where I devised a new scheme for coherent coupling between single organic molecules and dielectric waveguides. In August 2013, I moved to the Single-Molecule Optics group, led by Michel Orrit, at the Leiden institute of Physics and started working on tracing single electrons using single molecule spectroscopy. During my stay at Leiden, I also developed the nanocapillary electrophoretic tracking (nanoCET) technique with the goal of studying rapid changes in the electrophoretic mobility of single mobile nanoparticles.
In August 2015, together with Allard Mosk, we started a new research group named Physics of Light in Complex Systems,nanoLINX. In this group, I am the principle investigator for the research direction nanoElectroPhotonics.
My hobbies are rowing, listening to and making podcasts, and making Physics-related table-top demonstrations.
Practical information
- Informed public
- Free
Organizer
Contact
- Institute of Bioengineering (IBI, Aleksandra Radenovic)