Structural Biology in vivo by Electron Cryotomography
Event details
| Date | 22.08.2017 |
| Hour | 11:00 › 12:00 |
| Speaker | Yi-Wei Chang, Ph.D., California Institute of Technology, Pasadena, CA (USA) |
| Location | |
| Category | Conferences - Seminars |
BIOENGINEERING SEMINAR
Abstract:
Electron cryotomography (ECT) enables intact cells to be visualized in 3D in an essentially native state to macromolecular (~4 nm) resolution. It has allowed us to visualize the structures of macromolecular machines in their native context inside intact cells. In many cases, such machines cannot be purified intact for in vitro study. In other cases, the function of a structure is lost outside the cell, so that the mechanism can be understood only by observation in situ. In this presentation, I will use bacterial pilus and secretion systems as examples to show how ECT can help us to understand important biological processes by doing structural biology in vivo.
Bio:
Education:
National Tsing Hua University, Hsinchu, Taiwan
PhD, Structural Biology
2005 – 2010
Positions held:
Research Scientist
California Institute of Technology, Pasadena, CA (USA)
July 2014 – Present
HHMI Research Associate
Howard Hughes Medical Institute
July 2013 – June 2014
Postdoctoral Scholar
California Institute of Technology, Pasadena, CA (USA)
September 2011 – June 2013
Abstract:
Electron cryotomography (ECT) enables intact cells to be visualized in 3D in an essentially native state to macromolecular (~4 nm) resolution. It has allowed us to visualize the structures of macromolecular machines in their native context inside intact cells. In many cases, such machines cannot be purified intact for in vitro study. In other cases, the function of a structure is lost outside the cell, so that the mechanism can be understood only by observation in situ. In this presentation, I will use bacterial pilus and secretion systems as examples to show how ECT can help us to understand important biological processes by doing structural biology in vivo.
Bio:
Education:
National Tsing Hua University, Hsinchu, Taiwan
PhD, Structural Biology
2005 – 2010
Positions held:
Research Scientist
California Institute of Technology, Pasadena, CA (USA)
July 2014 – Present
HHMI Research Associate
Howard Hughes Medical Institute
July 2013 – June 2014
Postdoctoral Scholar
California Institute of Technology, Pasadena, CA (USA)
September 2011 – June 2013
Practical information
- Informed public
- Free
Organizer
Contact
- Institute of Bioengineering (IBI, Dietrich REINHARD)