Super-Resolution Microscopy with DNA Molecules
Event details
| Date | 08.03.2016 |
| Hour | 14:15 |
| Speaker | Ralf Jungmann, Ph.D., Max Planck Institute of Biochemistry, Munich (D) |
| Location | |
| Category | Conferences - Seminars |
BIOENGINEERING SEMINAR
Abstract:
Super-resolution fluorescence microscopy is a powerful tool for biological research, but obtaining multiplexed images for a large number of distinct target species in whole cells and beyond remains challenging. Here we use the transient binding of short fluorescently labeled oligonucleotides (DNA-PAINT, a variation of point accumulation for imaging in nanoscale topography) for simple and easy-to-implement multiplexed super-resolution imaging that achieves sub-10-nm spatial resolution in vitro on synthetic DNA structures.
We report a multiplexing approach (Exchange-PAINT) that allows sequential imaging of multiple targets using only a single dye and a single laser source. We experimentally demonstrate ten-color super-resolution imaging in vitro on synthetic DNA structures as well as four-color two-dimensional imaging and three-color 3D imaging of proteins in fixed cells.
Finally, we demonstrate whole cell imaging using DNA- and Exchange-PAINT and optical sectioning, now allowing DNA-based super-resolution imaging deep inside cells, away from the glass coverslip.
Bio:
Since 2014 Independent group leader at the Max Planck Institute of Biochemistry and Ludwig-Maximilians-Universität München
2011 - 2014 Postdoctoral researcher at the Wyss Institute for Biologically Inspired Engineering at Harvard University in the groups of Prof. Dr. Peng Yin and Prof. Dr. William M Shih
2010 - 2011 Postdoctoral researcher at Technische Universität München in the group of Prof. Dr. Friedrich C. Simmel
2007 - 2010 Ph.D. with Prof. Dr. Friedrich C. Simmel at Technische Universität München
2005 - 2006 Diploma Thesis work with Prof. Dr. Paul K Hansma at University of California Santa Barbara
2001 - 2006 Studies in physics at Saarland University
Abstract:
Super-resolution fluorescence microscopy is a powerful tool for biological research, but obtaining multiplexed images for a large number of distinct target species in whole cells and beyond remains challenging. Here we use the transient binding of short fluorescently labeled oligonucleotides (DNA-PAINT, a variation of point accumulation for imaging in nanoscale topography) for simple and easy-to-implement multiplexed super-resolution imaging that achieves sub-10-nm spatial resolution in vitro on synthetic DNA structures.
We report a multiplexing approach (Exchange-PAINT) that allows sequential imaging of multiple targets using only a single dye and a single laser source. We experimentally demonstrate ten-color super-resolution imaging in vitro on synthetic DNA structures as well as four-color two-dimensional imaging and three-color 3D imaging of proteins in fixed cells.
Finally, we demonstrate whole cell imaging using DNA- and Exchange-PAINT and optical sectioning, now allowing DNA-based super-resolution imaging deep inside cells, away from the glass coverslip.
Bio:
Since 2014 Independent group leader at the Max Planck Institute of Biochemistry and Ludwig-Maximilians-Universität München
2011 - 2014 Postdoctoral researcher at the Wyss Institute for Biologically Inspired Engineering at Harvard University in the groups of Prof. Dr. Peng Yin and Prof. Dr. William M Shih
2010 - 2011 Postdoctoral researcher at Technische Universität München in the group of Prof. Dr. Friedrich C. Simmel
2007 - 2010 Ph.D. with Prof. Dr. Friedrich C. Simmel at Technische Universität München
2005 - 2006 Diploma Thesis work with Prof. Dr. Paul K Hansma at University of California Santa Barbara
2001 - 2006 Studies in physics at Saarland University
Practical information
- Informed public
- Free