The physical epigenome: Quantitative approaches to characterize epigenetic systems
Event details
| Date | 02.02.2017 |
| Hour | 08:00 › 09:00 |
| Speaker | Dr. Courtney Hodges, Stanford University |
| Location | |
| Category | Conferences - Seminars |
Abstract:
Epigenetic regulatory mechanisms are critical for multicellular development and normal function. Indeed, the deregulation of these systems is a hallmark of cancer and other disorders. However, many essential regulatory events arise through short-lived interactions, which are difficult to characterize with conventional epigenetic approaches. To overcome this limitation, I have used novel physical and quantitative techniques to characterize epigenetic processes. In this talk, I will briefly describe three examples: (1) the use of single-molecule manipulation to reveal the dynamic barrier posed to RNA polymerase by nucleosomes; (2) integration of stochastic simulations with experiment to characterize heterochromatin formation and stability; and (3) applying quantitative epigenomics to reveal the mechanisms of ATP-dependent chromatin remodeling disrupted in cancer. These studies illuminate the physical basis for chromatin regulation, from detailed mechanisms of single molecules to global effects spanning the genome. My future research will integrate new "omic"-based strategies with single-molecule biophysical approaches, to reveal the quantitative principles governing epigenetic systems.
Epigenetic regulatory mechanisms are critical for multicellular development and normal function. Indeed, the deregulation of these systems is a hallmark of cancer and other disorders. However, many essential regulatory events arise through short-lived interactions, which are difficult to characterize with conventional epigenetic approaches. To overcome this limitation, I have used novel physical and quantitative techniques to characterize epigenetic processes. In this talk, I will briefly describe three examples: (1) the use of single-molecule manipulation to reveal the dynamic barrier posed to RNA polymerase by nucleosomes; (2) integration of stochastic simulations with experiment to characterize heterochromatin formation and stability; and (3) applying quantitative epigenomics to reveal the mechanisms of ATP-dependent chromatin remodeling disrupted in cancer. These studies illuminate the physical basis for chromatin regulation, from detailed mechanisms of single molecules to global effects spanning the genome. My future research will integrate new "omic"-based strategies with single-molecule biophysical approaches, to reveal the quantitative principles governing epigenetic systems.
Practical information
- Expert
- Free
- This event is internal
Organizer
- Prof. Benoit Deveaud, Institute of Physics
Contact
- Blandine Jérôme