Towards increased selectivity of cancer treatment by Photodynamic therapy
Event details
| Date | 31.05.2012 |
| Hour | 14:00 |
| Speaker | Prof. Pavol Miskovsky of Safarik University, Slovakia |
| Location | |
| Category | Conferences - Seminars |
Development of selective nano-delivery system and detection of therapeutically active form of drugs in cell
The efficacy of photosensitiers (pts) in inducing either apoptosis or necrosis after photodynamic action critically depends upon different parameters, including, in particular: i) selective pts accumulation in tumor cells and, ii) dynamics of sub-cellular distribution of therapeutically active form of drug.
Low-density lipoproteins (LDL) play a key role in the delivery of hydrophobic pts to tumor cells in PDT, including hypericin (Hyp). Construction and characterization of new LDL-based selective nano-delivery system is discussed in the presentation.
Dynamics of organelle specific sub-cellular redistribution of Hyp plays an important role in its photodynamic activity. In this presentation, we report the development and characterization of micrometer-sized dielectric beads with nano-metal structures attached to their surface. The metalized beads are sufficiently transparent enabling optical trapping while the presence of metal nano-islands provided the SERS. This highly efficient probe can be placed and scanned with nano-metric accuracy in living cells. Application of such optical nano-sensors is demonstrated on: i) detection of low quantities of photoactive drug emodine, (ii) study the kinetics of drug diffusion through the cellular membrane towards specific cell organelles.
The efficacy of photosensitiers (pts) in inducing either apoptosis or necrosis after photodynamic action critically depends upon different parameters, including, in particular: i) selective pts accumulation in tumor cells and, ii) dynamics of sub-cellular distribution of therapeutically active form of drug.
Low-density lipoproteins (LDL) play a key role in the delivery of hydrophobic pts to tumor cells in PDT, including hypericin (Hyp). Construction and characterization of new LDL-based selective nano-delivery system is discussed in the presentation.
Dynamics of organelle specific sub-cellular redistribution of Hyp plays an important role in its photodynamic activity. In this presentation, we report the development and characterization of micrometer-sized dielectric beads with nano-metal structures attached to their surface. The metalized beads are sufficiently transparent enabling optical trapping while the presence of metal nano-islands provided the SERS. This highly efficient probe can be placed and scanned with nano-metric accuracy in living cells. Application of such optical nano-sensors is demonstrated on: i) detection of low quantities of photoactive drug emodine, (ii) study the kinetics of drug diffusion through the cellular membrane towards specific cell organelles.
Practical information
- General public
- Free
Organizer
- Georges Wagnieres
Contact
- Georges Wagnieres