Making Use of 'Omics: From Systems Genetics to Cell Biology
Event details
| Date | 29.05.2017 |
| Hour | 13:30 |
| Speaker | Evan WILLIAMS ETH Zürich, Switzerland |
| Location | |
| Category | Conferences - Seminars |
SEMINAR SERIES : Trends in Physiology and Metabolism (Bio-682)
Abstract:
One of the fundamental tenants of biology tells us that DNA is transcribed into RNA, which is translated into protein, which affect the levels of the metabolites which carry out cellular actions. Microarrays and nucleotide sequencing technologies have made the measurement of DNA and RNA trivial, genome-wide, and accurate, yet the relationships between the layers of DNA, RNA, protein, and metabolites remain complex and unpredictable—i.e. measuring a gene's RNA level is of mediocre predictive value for its associated protein. Recently, mass spectrometry (MS)-based technologies have been refined to the point where quantitative, reliable, systems-scale measurements of the proteome and metabolome have become possible. Thus, recent systems biology studies have begun to focus on "multi-omics" studies—combining and comparing the data of the transcriptome, proteome, and metabolome and how these layers correspond to the phenotype of interest.
In this lecture, we will in particular examine how SWATH-MS, a next-generation proteomics technique, can be run across a large and diverse population to uncover new insights on mitochondrial protein localization, the stoichiometry of oxidative phosphorylation. With these new technologies, we can begin to develop and test novel hypotheses and design validation experiments that were previously infeasible.
Abstract:
One of the fundamental tenants of biology tells us that DNA is transcribed into RNA, which is translated into protein, which affect the levels of the metabolites which carry out cellular actions. Microarrays and nucleotide sequencing technologies have made the measurement of DNA and RNA trivial, genome-wide, and accurate, yet the relationships between the layers of DNA, RNA, protein, and metabolites remain complex and unpredictable—i.e. measuring a gene's RNA level is of mediocre predictive value for its associated protein. Recently, mass spectrometry (MS)-based technologies have been refined to the point where quantitative, reliable, systems-scale measurements of the proteome and metabolome have become possible. Thus, recent systems biology studies have begun to focus on "multi-omics" studies—combining and comparing the data of the transcriptome, proteome, and metabolome and how these layers correspond to the phenotype of interest.
In this lecture, we will in particular examine how SWATH-MS, a next-generation proteomics technique, can be run across a large and diverse population to uncover new insights on mitochondrial protein localization, the stoichiometry of oxidative phosphorylation. With these new technologies, we can begin to develop and test novel hypotheses and design validation experiments that were previously infeasible.
Links
Practical information
- Informed public
- Free
Organizer
- Prof. Lluis Fajas (UNIL), Prof. Kei Sakamoto (NIHS) and Prof. Kristina Schoonjans (EPFL)
Contact
- Prof. Kristina Schoonjans (EPFL) - [email protected]