An evolutionary switch in J-protein biology affects prokaryotic and eukaryotic protein disaggregation
Event details
| Date | 15.11.2016 |
| Hour | 14:15 › 16:00 |
| Speaker | Nadinath Nillegoda, Heidelberg University, Germany |
| Location | |
| Category | Conferences - Seminars |
BIOLOGICAL & STATISTICAL PHYSICS SEMINAR
Abstract:
Decreased cellular capacity in protein aggregate clearance manifests in cellular deterioration, aging and disease. Toxic intracellular aggregates formed by misfolded proteins are reversed and/or limited by multi-tiered cellular quality control systems.
We recently reported J-proteins of classes A and B cooperate via interclass complex formation to mediate substrate specificity of Hsp70-based aggregate solubilizing systems (disaggregases) in metazoa. What remains unclear is whether these mixed class J-protein complexes occur also in non-metazoans given the fact that orthologs of both classes exist in bacteria, fungi, plant and protozoa. Using a broad set of experimental approaches, we find a switching in J-protein biology at the prokaryote-to-eukaryote transition where class members network allowing for the emergence of powerful, yet regulatable eukaryotic disaggregase systems.
We also describe a naturally occurring strategy to correctly pair J-proteins of different types, ensuing functional integrity within networks in expanded J-protein families during rise of complex life.
Abstract:
Decreased cellular capacity in protein aggregate clearance manifests in cellular deterioration, aging and disease. Toxic intracellular aggregates formed by misfolded proteins are reversed and/or limited by multi-tiered cellular quality control systems.
We recently reported J-proteins of classes A and B cooperate via interclass complex formation to mediate substrate specificity of Hsp70-based aggregate solubilizing systems (disaggregases) in metazoa. What remains unclear is whether these mixed class J-protein complexes occur also in non-metazoans given the fact that orthologs of both classes exist in bacteria, fungi, plant and protozoa. Using a broad set of experimental approaches, we find a switching in J-protein biology at the prokaryote-to-eukaryote transition where class members network allowing for the emergence of powerful, yet regulatable eukaryotic disaggregase systems.
We also describe a naturally occurring strategy to correctly pair J-proteins of different types, ensuing functional integrity within networks in expanded J-protein families during rise of complex life.
Practical information
- Informed public
- Free
Organizer
- Prof. Paolo De Los Rios, Laboratory of Statistical Biophysics, Institute of Physic and Institute of Bioengineering
Contact
- Céline Burkhard, Laboratory of Statistical Biophysics (LBS)