Bioimaging at the nanoscale: Single-molecule and super-resolution fluorescence
Event details
| Date | 20.09.2013 |
| Hour | 14:00 › 15:30 |
| Speaker | Pr. Xiaowei Zhuang (Harvard) |
| Location | |
| Category | Conferences - Seminars |
Dissecting the inner workings of a cell requires imaging methods with chemical specificity, single-molecule sensitivity, molecular-scale resolution, and dynamic imaging capability such that molecular interactions inside the cell can be directly visualized. Fluorescence microscopy is a powerful imaging modality for cell biological research largely owning to its molecular specificity and dynamic imaging capability. However, the spatial resolution of light microscopy, classically limited by diffraction to a few hundred nanometers, is substantially larger than typical molecular length scales in cells. Hence many subcellular structures and dynamics cannot be resolved by conventional fluorescence microscopy. We recently developed a super-resolution fluorescence microscopy method, stochastic optical reconstruction microscopy (STORM), which has allowed fluorescence imaging with molecular-scale resolution and enabled discoveries of novel sub-cellular structures. In this talk, I will describe STORM and other singlemolecule imaging methods explored in the lab, focusing on recent biological discoveries enabled by these high-resolution imaging approaches.
Practical information
- General public
- Free