<b>“The road to animal-wide relative quantification of proteins by SILAC and LC-MS”</b>
Event details
| Date | 15.01.2009 |
| Hour | 12:15 |
| Speaker | Dr.Marcus Krüger, Max Planck Institute for Heart and Lung Research (Bad Nauheim, Germany) |
| Location | |
| Category | Conferences - Seminars |
This seminar is part of the regular series of IBI cross-disciplinary seminars and is co-organized by Prof. Auwerx group and the EPFL-Proteomics Core Facility.
Short Abstract: SILAC (stable isotope labeling of amino acids in cell culture) has unique advantages for quantitative and functional proteomics because of its inherent accuracy of quantitation and the ease of interpretation of mass-spectrometry (MS) results. In a SILAC experiment two cell populations are generated, one in a medium that contains the natural amino acid (i.e. 12C6-lysine) and the other in a medium that contains the heavy isotope-substituted version (i.e. 13C6-lysine). This allows direct comparison of protein expression levels by mixing the non-labeled ‘light’ and labeled ‘heavy’ cell populations. To date SILAC-labeling has been focused mainly to cell culture or microorganisms. I will talk about whether and to what extent whole mice can be labeled by SILAC. Furthermore, I will talk about the estimation of protein turnover rates and the analysis of knock-out mouse proteomes. The focus will be on the analysis of blood cells lacking expression of b1 integrin or integrin tail binding protein Kindlin-3. I will show that the absence of Kindlin-3 results in structural defects of erythrocytes.
The seminar is a unique opportunity to learn about what modern quantitative proteomics techniques like SILAC can bring to the study of knock-out mouse phenotypes at the protein expression level.
People interested in meeting Dr. Krüger in the afternoon of the 15th or on the 16th are welcome to contact Marc Moniatte or Prof. Auwerx.
Links
Practical information
- General public
- Free
Contact
- Dr. Marc Moniatte ou Prof. Johan Auwerx