"Differentiation of ES cells into pancreatic and hepatic cells"
Event details
| Date | 13.07.2009 |
| Hour | 12:15 |
| Speaker | Prof. Shoen Kume, Kumamoto University, Department of Stem Cell Biology |
| Location |
SV 1717A
|
| Category | Conferences - Seminars |
The endoderm gives rise to the respiratory and digestive organs such as lung, liver, pancreas and intestine, which are therapeutically useful. Here I show that ES cells cultured on M15 cells, a supportive cell line of mesonephros origin, are efficiently differentiated into an endodermal fate, and then adopted fates of various digestive organs such as pancreatic and hepatic lineages. Under culture condition for pancreatic differentiation, approximately 31% of the ES cells were induced to differentiate to Pdx1-expressing cells. Transplantation studies demonstrate the potential of these all pancreatic lineages, such as endocrine, exocrinePdx1+ cells to turn into and duct cells. Under condition for hepatic differentiation, the expression of AFP-, Albumin-, DBA- positive cells appeared in a sequential manner, and other markers associated with mature hepatic cells appeared after a prolonged culture.
Using a synthesized basement membrane (sBM) substratum and the modification of culture media, ES cells could be selectively differentiated into a pancreatic fate. IPS cells could also be induced to pancreatic cells using the same protocol as ES cells upon culture on sBM substrates. These results suggest that basement membrane play a role to promote differentiation.
In conclusion, we have established an in vitro differentiation procedure to direct ES cell differentiation into pancreatic and hepatic lineages. This novel procedure for endoderm differentiation will be useful for elucidation of molecular mechanisms of gut regionalization and could represent an attractive approach for a surrogate cell source for not only regenerative medicine, but also pharmaceutical studies such as toxicology.
Practical information
- General public
- Free
Contact
- Anne Grapin-Botton