EPFL BioE Talks SERIES "A Novel Neuroimmune Human Brain Organoid Model to Study Microglia in Health and Disease"
Event details
| Date | 30.10.2023 |
| Hour | 16:00 › 17:00 |
| Speaker | Prof. Abed A. Mansour, Department of Medical Neurobiology, The Institute for Medical Research (IMRIC), Faculty of Medicine, The Hebrew University of Jerusalem (IL) |
| Location | Online |
| Category | Conferences - Seminars |
| Event Language | English |
WEEKLY EPFL BIOE TALKS SERIES
Abstract:
Microglia are specialized brain-resident macrophages that play crucial roles in brain development, homeostasis, and disease. However, until now, the ability to model interactions between the human brain environment and microglia has been severely limited. To overcome these limitations, we developed an in vivo xenotransplantation approach that allows us to study functionally mature human microglia (hMGs) that operate within a physiologically relevant, vascularized immunocompetent human brain organoid (iHBO) model. Our data show that organoid-resident hMGs gain human-specific transcriptomic signatures that closely resemble their in vivo counterparts. In vivo two-photon imaging reveals that hMGs actively engage in surveilling the human brain environment, react to local injuries, and respond to systemic inflammatory cues. Finally, we demonstrate that the transplanted iHBOs developed here offer the unprecedented opportunity to study functional human microglia phenotypes in health and disease and provide experimental evidence for a brain-environment-induced immune response in a patient-specific model of autism with macrocephaly.
Bio:
Education:
2005 - 2011
Ph.D. in Neuro-Developmental Genetics
Supervisor: Prof. Nissim Ben-Arie
Dept. of Cell and Developmental Biology, Hebrew university of Jerusalem (IL)
2000 - 2003
MSc, Medical Science
Hebrew University-Hadassah Medical School (IL)
1997 - 2000
BSc, Biology
The Hebrew University of Jerusalem (IL)
Experience:
Jan 2021 - Present
Assistant Professor
Dept. Medical Neurobiology, Institute for Medical Research
Faculty of Medicine-Hebrew University of Jerusalem (IL)
Feb 2015 - Present
Postdoctoral Research Associate
Fred H. Gage Lab, Salk Institute for Biological Studies, La Jolla, CA (USA)
Dec 2011 - Jan 2015
Postdoctoral Research Fellow
Dr. Jacob (Yaqub) Hanna Lab, Laboratory for Pluripotent Cell Studies
Dept. of Molecular Genetics, Weizmann Institute of Science (IL)
Zoom link (with one-time registration for the whole series) for attending remotely: https://go.epfl.ch/EPFLBioETalks
Instructions for 1st-year Ph.D. students who are under EDBB’s mandatory seminar attendance rule:
IF you are not attending in-person in the room, please make sure to
Abstract:
Microglia are specialized brain-resident macrophages that play crucial roles in brain development, homeostasis, and disease. However, until now, the ability to model interactions between the human brain environment and microglia has been severely limited. To overcome these limitations, we developed an in vivo xenotransplantation approach that allows us to study functionally mature human microglia (hMGs) that operate within a physiologically relevant, vascularized immunocompetent human brain organoid (iHBO) model. Our data show that organoid-resident hMGs gain human-specific transcriptomic signatures that closely resemble their in vivo counterparts. In vivo two-photon imaging reveals that hMGs actively engage in surveilling the human brain environment, react to local injuries, and respond to systemic inflammatory cues. Finally, we demonstrate that the transplanted iHBOs developed here offer the unprecedented opportunity to study functional human microglia phenotypes in health and disease and provide experimental evidence for a brain-environment-induced immune response in a patient-specific model of autism with macrocephaly.
Bio:
Education:
2005 - 2011
Ph.D. in Neuro-Developmental Genetics
Supervisor: Prof. Nissim Ben-Arie
Dept. of Cell and Developmental Biology, Hebrew university of Jerusalem (IL)
2000 - 2003
MSc, Medical Science
Hebrew University-Hadassah Medical School (IL)
1997 - 2000
BSc, Biology
The Hebrew University of Jerusalem (IL)
Experience:
Jan 2021 - Present
Assistant Professor
Dept. Medical Neurobiology, Institute for Medical Research
Faculty of Medicine-Hebrew University of Jerusalem (IL)
Feb 2015 - Present
Postdoctoral Research Associate
Fred H. Gage Lab, Salk Institute for Biological Studies, La Jolla, CA (USA)
Dec 2011 - Jan 2015
Postdoctoral Research Fellow
Dr. Jacob (Yaqub) Hanna Lab, Laboratory for Pluripotent Cell Studies
Dept. of Molecular Genetics, Weizmann Institute of Science (IL)
Zoom link (with one-time registration for the whole series) for attending remotely: https://go.epfl.ch/EPFLBioETalks
Instructions for 1st-year Ph.D. students who are under EDBB’s mandatory seminar attendance rule:
IF you are not attending in-person in the room, please make sure to
- send D. Reinhard a note before noon on seminar day, informing that you plan to attend the talk online, and
- be signed in on Zoom with a recognizable user name (not a pseudonym making it difficult or impossible to be identified).
Practical information
- Informed public
- Registration required
Organizer
- Prof. Hilal Lashuel, EPFL
Contact
- Marie Rodriguez and Institute of Bioengineering (IBI), Dietrich REINHARD