EPFL BioE Talks SERIES "Advanced Concepts of Super-Resolution Fluorescence Microscopy"
Event details
| Date | 22.04.2024 |
| Hour | 12:15 › 13:15 |
| Speaker | Prof. Jörg Enderlein, Third Institute of Physics – Biophysics, Georg August University, Göttingen (DE) |
| Location | Online |
| Category | Conferences - Seminars |
| Event Language | English |
WEEKLY EPFL BIOE TALKS SERIES (sandwiches provided)
Abstract:
With the advent of super-resolution microscopy, the last ~25 years have seen a revolution in optical microscopy, pushing the spatial resolution capabilities of optical microscopy towards length scales that were typically accessible only by electron microscopy. In my presentation, I will give a short overview of the different principal approaches to super-resolution microscopy. Then, I will focus on two specific techniques where our group has contributed most. The first is Image Scanning Microscopy or ISM [1-3]. This technique uses a simple combination of confocal microscopy with wide-field image detection for doubling the resolution of conventional microscopy. I will explain the physical principals behind ISM, and the various kinds of its implementation. Meanwhile, ISM has found broad and wide applications and lies behind state-of-the-art commercial systems such as the extremely successful AiryScan microscope from Carl Zeiss Jena. The second method is Metal-and Graphene-Induced Energy Transfer imaging or MIET/GIET imaging [4-6]. It addresses the axial resolution in microscopy, which is particularly important for resolving three-dimensional structures. MIET/GIET is based on the intricate electrodynamic interaction of fluorescent emitters with metallic nanostructures. I will present the basic principles and several applications of this technique.
References:
1. Müller, C. B., & Enderlein, J. (2010). Image scanning microscopy. Phys. Rev. Lett. 104, 198101.
2. Schulz, O., Pieper, C., Clever, M., Pfaff, J., Ruhlandt, A., Kehlenbach, R. H., ... & Enderlein, J. (2013). Resolution doubling in fluorescence microscopy with confocal spinning-disk image scanning microscopy. PNAS 110, 21000-21005.
3. Gregor, I., Spiecker, M., Petrovsky, R., Großhans, J., Ros, R., & Enderlein, J. (2017). Rapid nonlinear image scanning microscopy. Nature Methods 14, 1087.
4. Chizhik, A. I., Rother, J., Gregor, I., Janshoff, A., & Enderlein, J. (2014). Metal-induced energy transfer for live cell nanoscopy. Nature Photonics 8, 124.
5. Ghosh, A., Sharma, A., Chizhik, A. I., Isbaner, S., Ruhlandt, D., Tsukanov, R., ... & Enderlein, J. (2019). Graphene-based metal-induced energy transfer for sub-nanometre optical localization. Nature Photonics 13, 860-865.
6. Ghosh, A., Chizhik, A. I., Karedla, N., & Enderlein, J. (2021). Graphene-and metal-induced energy transfer for single-molecule imaging and live-cell nanoscopy with (sub)-nanometer axial resolution. Nature Protocols 16, 3695-3715.
7. Chen, T., Ghosh, A., & Enderlein, J. (2023). Cholesterol-induced nanoscale variations in the thickness of phospholipid membranes. Nano Letters 23, 2421-2426.
Bio:
Jörg Enderlein has studied physics in Odessa (Ukraine) between 1981 and 1986. He obtained his PhD at Humboldt-University in Berlin (Germany) in 1991 for his research on non-linear reaction diffusion systems. After his PhD, he joined PicoQuant GmbH in berlin as a research scientist, where he was involved in the development of technology for single-molecule fluorescence detection and spectroscopy. After his PostDoc with the group of Dick Keller at the Los Alamos National Laboratory (USA), he became an assistant professor at Regensburg University (Germany) in 1997. In 2001, he became a Heisenberg Fellow of the German Research Council and a group leader at the Forschungszentrum Jülich, Germany’s largest research institution. In 2007, he became full professor for Biophysical Chemistry at the Eberhard Karls University in Tübingen, and since 2008, he is full professor for Biophysics at the Georg August University in Göttingen. His research interests are single-molecule fluorescence spectroscopy, super-resolution fluorescence microscopy, and nano-optics and plasmonics.
Zoom link (with one-time registration for the whole series) for attending remotely: https://go.epfl.ch/EPFLBioETalks
Instructions for 1st-year Ph.D. students who are under EDBB’s mandatory seminar attendance rule:
IN CASE you cannot attend in-person in the room, please make sure to
Abstract:
With the advent of super-resolution microscopy, the last ~25 years have seen a revolution in optical microscopy, pushing the spatial resolution capabilities of optical microscopy towards length scales that were typically accessible only by electron microscopy. In my presentation, I will give a short overview of the different principal approaches to super-resolution microscopy. Then, I will focus on two specific techniques where our group has contributed most. The first is Image Scanning Microscopy or ISM [1-3]. This technique uses a simple combination of confocal microscopy with wide-field image detection for doubling the resolution of conventional microscopy. I will explain the physical principals behind ISM, and the various kinds of its implementation. Meanwhile, ISM has found broad and wide applications and lies behind state-of-the-art commercial systems such as the extremely successful AiryScan microscope from Carl Zeiss Jena. The second method is Metal-and Graphene-Induced Energy Transfer imaging or MIET/GIET imaging [4-6]. It addresses the axial resolution in microscopy, which is particularly important for resolving three-dimensional structures. MIET/GIET is based on the intricate electrodynamic interaction of fluorescent emitters with metallic nanostructures. I will present the basic principles and several applications of this technique.
References:
1. Müller, C. B., & Enderlein, J. (2010). Image scanning microscopy. Phys. Rev. Lett. 104, 198101.
2. Schulz, O., Pieper, C., Clever, M., Pfaff, J., Ruhlandt, A., Kehlenbach, R. H., ... & Enderlein, J. (2013). Resolution doubling in fluorescence microscopy with confocal spinning-disk image scanning microscopy. PNAS 110, 21000-21005.
3. Gregor, I., Spiecker, M., Petrovsky, R., Großhans, J., Ros, R., & Enderlein, J. (2017). Rapid nonlinear image scanning microscopy. Nature Methods 14, 1087.
4. Chizhik, A. I., Rother, J., Gregor, I., Janshoff, A., & Enderlein, J. (2014). Metal-induced energy transfer for live cell nanoscopy. Nature Photonics 8, 124.
5. Ghosh, A., Sharma, A., Chizhik, A. I., Isbaner, S., Ruhlandt, D., Tsukanov, R., ... & Enderlein, J. (2019). Graphene-based metal-induced energy transfer for sub-nanometre optical localization. Nature Photonics 13, 860-865.
6. Ghosh, A., Chizhik, A. I., Karedla, N., & Enderlein, J. (2021). Graphene-and metal-induced energy transfer for single-molecule imaging and live-cell nanoscopy with (sub)-nanometer axial resolution. Nature Protocols 16, 3695-3715.
7. Chen, T., Ghosh, A., & Enderlein, J. (2023). Cholesterol-induced nanoscale variations in the thickness of phospholipid membranes. Nano Letters 23, 2421-2426.
Bio:
Jörg Enderlein has studied physics in Odessa (Ukraine) between 1981 and 1986. He obtained his PhD at Humboldt-University in Berlin (Germany) in 1991 for his research on non-linear reaction diffusion systems. After his PhD, he joined PicoQuant GmbH in berlin as a research scientist, where he was involved in the development of technology for single-molecule fluorescence detection and spectroscopy. After his PostDoc with the group of Dick Keller at the Los Alamos National Laboratory (USA), he became an assistant professor at Regensburg University (Germany) in 1997. In 2001, he became a Heisenberg Fellow of the German Research Council and a group leader at the Forschungszentrum Jülich, Germany’s largest research institution. In 2007, he became full professor for Biophysical Chemistry at the Eberhard Karls University in Tübingen, and since 2008, he is full professor for Biophysics at the Georg August University in Göttingen. His research interests are single-molecule fluorescence spectroscopy, super-resolution fluorescence microscopy, and nano-optics and plasmonics.
Zoom link (with one-time registration for the whole series) for attending remotely: https://go.epfl.ch/EPFLBioETalks
Instructions for 1st-year Ph.D. students who are under EDBB’s mandatory seminar attendance rule:
IN CASE you cannot attend in-person in the room, please make sure to
- send D. Reinhard a note well ahead of time (ideally before seminar day), informing that you plan to attend the talk online, and, during seminar:
- be signed in on Zoom with a recognizable user name (not any alias making it difficult or impossible to identify you).
Practical information
- Informed public
- Registration required
Organizer
Contact
- Institute of Bioengineering (IBI), Dietrich REINHARD