EPFL BioE Talks SERIES "High-Throughput Super-Resolution Microscopy for Reconstructing Molecular Architecture"


Event details

Date 09.11.2020
Hour 16:3017:00
Speaker Prof. Suliana Manley, Institute of Physics and Institute of Bioengineering, EPFL, Lausanne (CH)
Location Online
Category Conferences - Seminars

(note that this talk is number two of a double-feature seminar - see details of the first talk here)

Super-resolution microscopy is an important tool because it enables imaging beyond the diffraction limit, while maintaining the specificity and live-cell possibilities of fluorescence microscopy. Yet, many important biological questions remain out of reach due to challenges in acquiring and analyzing statistically significant datasets. We created a uniform illumination scheme to enable high quality, large field of view PALM/STORM, and now iSIM images. We demonstrate the power of this approach for studying large, multi-protein complexes within cells, using the centriole as a case study. To study the organization of such complexes, particle-based analysis has proven to be powerful, but has been limited so far by difficulties in generating large multi-color particle libraries, as well as the complexity of orientational alignment. We have addressed both challenges and, as a result, present a framework for deciphering the 3D organization of protein complexes composed of multiple components.

Born in the United States, Suliana Manley studied at Rice University where she obtained a bachelor’s degree in physics and mathematics in 1997, cum laude. She continued her studies at Harvard University, where she earned a PhD in physics under the supervision of Prof. Dave Weitz in 2004. After this, she conducted postdoctoral research on lipid bilayer and red blood cell membrane dynamics at MIT. She subsequently went on to work as a postdoctoral researcher in the cell biology laboratory of Jennifer Lippincott-Schwartz at the National Institutes of Health (USA). During this time, she developed a highly promising optical method (sptPALM) for studying the dynamics of large ensembles of single proteins in membranes and inside cells. She became a tenure-track assistant professor of physics at the EPFL in 2009, and was promoted to associate professor with tenure in 2016.

Zoom link (with registration) for attending remotely: https://go.epfl.ch/EPFLBioETalks

IMPORTANT NOTICE: due to restrictions resulting from the ongoing Covid-19 situation, this seminar can be followed via Zoom web-streaming only, following prior one-time registration through the link above.

Practical information

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