High-resolution lensless microscopy and nonlinear imaging: new tools for biomedical applications
Event details
| Date | 22.05.2014 |
| Hour | 16:30 › 17:30 |
| Speaker | Dr Stefan Witte Free, University of Amsterdam |
| Location | |
| Category | Conferences - Seminars |
The ability to image biological objects at the sub-cellular level is of major importance for life science and clinical diagnostics. Especially in neuroscience, label-free 3D microscopic imaging modalities are highly needed.
In this talk, I will present our research efforts on third-harmonic generation (THG) microscopy, which we have shown to be a powerful technique for live brain imaging with sub-cellular resolution [1]. THG microscopy allows high-resolution structural imaging of grey and white matter as well as red blood cells with good contrast, and even enables guided patch clamping deep inside brain tissue.
In the quest towards ever higher resolution, we have recently started a research project on soft-X-ray imaging, with the goal of single-cell imaging at ultrahigh resolution, using a compact table-top setup based on high-harmonic generation. As this is a wavelength range where good-quality imaging optics are problematic, we are working on the development of high-resolution lensless imaging techniques. I will discuss our results on lensless diffractive imaging with ultra-broadband sources [2], as well as our progress towards soft-X-ray imaging.
[1]S. Witte, A. Negrean, J.C. Lodder, C.P.J. de Kock, G.T. Silva, H.D. Mansvelder, M.L. Groot, Proc. Natl. Acad. Sci. USA 108, 5970 (2011).
[2] S. Witte, V.T. Tenner, D.W.E. Noom, K.S.E. Eikema, Light: Sci. Appl. 3, e163 (2014).
In this talk, I will present our research efforts on third-harmonic generation (THG) microscopy, which we have shown to be a powerful technique for live brain imaging with sub-cellular resolution [1]. THG microscopy allows high-resolution structural imaging of grey and white matter as well as red blood cells with good contrast, and even enables guided patch clamping deep inside brain tissue.
In the quest towards ever higher resolution, we have recently started a research project on soft-X-ray imaging, with the goal of single-cell imaging at ultrahigh resolution, using a compact table-top setup based on high-harmonic generation. As this is a wavelength range where good-quality imaging optics are problematic, we are working on the development of high-resolution lensless imaging techniques. I will discuss our results on lensless diffractive imaging with ultra-broadband sources [2], as well as our progress towards soft-X-ray imaging.
[1]S. Witte, A. Negrean, J.C. Lodder, C.P.J. de Kock, G.T. Silva, H.D. Mansvelder, M.L. Groot, Proc. Natl. Acad. Sci. USA 108, 5970 (2011).
[2] S. Witte, V.T. Tenner, D.W.E. Noom, K.S.E. Eikema, Light: Sci. Appl. 3, e163 (2014).
Practical information
- General public
- Free
Organizer
- Dr Frank van Mourik