How antibiotics work: a metabolomics approach
Event details
| Date | 02.03.2016 |
| Hour | 11:00 › 12:00 |
| Speaker | Dr. Luiz Pedro de Carvahlo, The Francis Crick Institute, London, UK |
| Location | |
| Category | Conferences - Seminars |
Timely and precise identification of the target(s) of biologically active small molecules remains a great challenge in modern drug discovery. Importantly, rigorous assessment of in vivo target inhibition (target engagement) has emerged as an important parameter during drug development campaigns. In this presentation we demonstrate the power of enzymology combined with metabolomics to identify the exact metabolic target inhibited by –chloro-D-alanine (BCDA), a canonical alanine racemase inhibitor. Using a combination of enzymology and metabolomics we reveal that BCDA kills Mycobacterium tuberculosis by a completely new mechanism. Stable isotope labelling coupled to metabolomics allow direct assessment of target engagement by BCDA, allowing direct monitoring of enzyme and pathway inhibition.
Practical information
- Informed public
- Free
Organizer
- Unité UPCOL
Contact
- Prof. Stewart Cole