Novel strategies to design microbial production strains - ready to be scaled up
Event details
| Date | 16.10.2015 |
| Hour | 16:15 |
| Speaker | Prof. Anton Glieder, Technische Universität Graz, Austria |
| Location | |
| Category | Conferences - Seminars |
Anton Glieder's main interests are in protein engineering, yeast-based protein expression and whole-cell catalysis systems, directed evolution, industrial biocatalysis, yeast metabolic engineering and synthetic biology. His current interests specifically focus on two main topics:
(i) the engineering of proteins towards improved fitness for industrial or therapeutic application, and
(ii) using systems biology and metabolic engineering to improve the physiology of expression strains to enhance production of proteins as well as primary and secondary metabolites.
In addition to the application-oriented engineering of proteins, evolutionary engineering techniques are attractive for clarifying the structure-function relationship of enzymes and their cellular functions.
Laboratory Techniques:
Protein engineering methods are used routinely in A. Glieder's laboratory for rational design as well as for the random generation of large libraries of individual variants of industrial enzymes from prokaryotic and eukaryotic cells. Enzyme assays in high-throughput formats are well established for many different enzyme families, allowing qualitative analysis on a colony level as well as paralleled kinetic analysis of large numbers of enzyme variants. High-throughput LC-MS based analytics was established for screenings using unmodified target substrates instead of surrogate substrates. Special proprietary tools for efficient protein expression and engineering employing Pichia pastoris were developed.
Bio: Anton Glieder studied Chemistry and Biochemistry at the University of Vienna. After receiving his diploma, he moved to Graz and completed his PhD in microbiology at the University of Graz. From 1993 to 1997, he was a postdoctoral fellow at the special research programme Biocatalysis at the Graz University of Technology. Following 3 years of industrial experience, he started his academic career as an assistant professor at the Graz University of Technology, and after a year, as a visiting scientist in Frances H. Arnold´s lab at Caltech.
He joined the Research Centre Applied Biocatalysis (A-B) as a project leader and key researcher in 2002. In 2003, Anton Glieder became associate professor at the Institute of Molecular Biotechnology at the Graz University of Technology, obtaining the first Dr. W. Houska award in 2006. In 2008, Anton Glieder became scientific director of the ACIB initiative, and in 2009, he also became the scientific director of the Research Centre Applied Biocatalysis (Angewandte Biokatalyse GmbH). As of November 1, 2010, he was part of the Executive Board of Directors (Scientific Director . CEO & CSO) of the Austrian Centre of Industrial Biotechnology (ACIB GmbH), where he remained until March 2014.
Currently, he is pursuing his research as a professor at the Graz University of Technology and an ACIB key scientist.
(i) the engineering of proteins towards improved fitness for industrial or therapeutic application, and
(ii) using systems biology and metabolic engineering to improve the physiology of expression strains to enhance production of proteins as well as primary and secondary metabolites.
In addition to the application-oriented engineering of proteins, evolutionary engineering techniques are attractive for clarifying the structure-function relationship of enzymes and their cellular functions.
Laboratory Techniques:
Protein engineering methods are used routinely in A. Glieder's laboratory for rational design as well as for the random generation of large libraries of individual variants of industrial enzymes from prokaryotic and eukaryotic cells. Enzyme assays in high-throughput formats are well established for many different enzyme families, allowing qualitative analysis on a colony level as well as paralleled kinetic analysis of large numbers of enzyme variants. High-throughput LC-MS based analytics was established for screenings using unmodified target substrates instead of surrogate substrates. Special proprietary tools for efficient protein expression and engineering employing Pichia pastoris were developed.
Bio: Anton Glieder studied Chemistry and Biochemistry at the University of Vienna. After receiving his diploma, he moved to Graz and completed his PhD in microbiology at the University of Graz. From 1993 to 1997, he was a postdoctoral fellow at the special research programme Biocatalysis at the Graz University of Technology. Following 3 years of industrial experience, he started his academic career as an assistant professor at the Graz University of Technology, and after a year, as a visiting scientist in Frances H. Arnold´s lab at Caltech.
He joined the Research Centre Applied Biocatalysis (A-B) as a project leader and key researcher in 2002. In 2003, Anton Glieder became associate professor at the Institute of Molecular Biotechnology at the Graz University of Technology, obtaining the first Dr. W. Houska award in 2006. In 2008, Anton Glieder became scientific director of the ACIB initiative, and in 2009, he also became the scientific director of the Research Centre Applied Biocatalysis (Angewandte Biokatalyse GmbH). As of November 1, 2010, he was part of the Executive Board of Directors (Scientific Director . CEO & CSO) of the Austrian Centre of Industrial Biotechnology (ACIB GmbH), where he remained until March 2014.
Currently, he is pursuing his research as a professor at the Graz University of Technology and an ACIB key scientist.
Practical information
- General public
- Free
Organizer
- Jeremy Luterbacher