Quantitative Time-Resolved Dissection of Gene Expression and Cell Identity
Event details
Date | 06.11.2019 |
Hour | 12:15 › 13:15 |
Speaker | Prof. David Suter, Institute of Bioengineering, EPFL (CH) |
Location | |
Category | Conferences - Seminars |
Abstract:
The genome contains the building plan of living organisms and largely determines their phenotype. Within complex multicellular animals, the differential activity of a common set of genes allows generating a large diversity of cell types. However, the activity of individual genes displays large temporal fluctuations, and mechanisms of gene regulation are disrupted when cells enter into mitosis. How these perturbations impact the control of cell identity is not understood.
My laboratory develops new approaches to dissect gene expression and cell fate decisions in a quantitative and time-resolved manner. I will first describe our quantifications of transcription factor-DNA interactions, the memory of gene activity through cell generations, and the coordination of protein synthesis and degradation in single living cells. I will then discuss how temporal fluctuations of transcription factor levels and their activity across the cell cycle impact self-renewal and differentiation of embryonic stem cells. Our work illustrates how quantitative time-resolved analysis of gene expression allows deepening our understanding of cell identity control.
Short bio:
David Suter obtained his MD/PhD at the University of Geneva on stem cell biology, followed by a first postdoctoral training with Ueli Schibler focusing on single cell monitoring of transcription. He then joined the group of Sunney Xie at Harvard University, where he co-developed a new method for single molecule live imaging of transcription factors in mammalian cells. In 2013, he obtained a Swiss National Science Foundation Professorship and was subsequently nominated Tenure Track assistant Professor at the Institute of Bioengineering and EPFL School of Life Sciences. His research focuses on quantitative approaches to study gene expression and developmental cell fate decisions.
Zoom link for attending remotely: https://epfl.zoom.us/j/143970197
The genome contains the building plan of living organisms and largely determines their phenotype. Within complex multicellular animals, the differential activity of a common set of genes allows generating a large diversity of cell types. However, the activity of individual genes displays large temporal fluctuations, and mechanisms of gene regulation are disrupted when cells enter into mitosis. How these perturbations impact the control of cell identity is not understood.
My laboratory develops new approaches to dissect gene expression and cell fate decisions in a quantitative and time-resolved manner. I will first describe our quantifications of transcription factor-DNA interactions, the memory of gene activity through cell generations, and the coordination of protein synthesis and degradation in single living cells. I will then discuss how temporal fluctuations of transcription factor levels and their activity across the cell cycle impact self-renewal and differentiation of embryonic stem cells. Our work illustrates how quantitative time-resolved analysis of gene expression allows deepening our understanding of cell identity control.
Short bio:
David Suter obtained his MD/PhD at the University of Geneva on stem cell biology, followed by a first postdoctoral training with Ueli Schibler focusing on single cell monitoring of transcription. He then joined the group of Sunney Xie at Harvard University, where he co-developed a new method for single molecule live imaging of transcription factors in mammalian cells. In 2013, he obtained a Swiss National Science Foundation Professorship and was subsequently nominated Tenure Track assistant Professor at the Institute of Bioengineering and EPFL School of Life Sciences. His research focuses on quantitative approaches to study gene expression and developmental cell fate decisions.
Zoom link for attending remotely: https://epfl.zoom.us/j/143970197
Practical information
- General public
- Free
Organizer
- SV Faculty
Contact
- M. Mary, Dr H. Hirling