Scanning Ion Conductance Microscopy of Soft Biological Matter
Event details
| Date | 04.05.2015 |
| Speaker | Prof. Yuri Korchev, Imperial College London |
| Location | |
| Category | Conferences - Seminars |
Molecular Biology has advanced we know much about the individual molecular components that make up living cells down to the level of the individual atoms. The challenge, however, is to fully understand the functional integration of these components. This requires determining how the molecular machines that make up a living cell are organized and interact together not at the atomic length scale but on a nm scale. To do this we need to develop and applying nanoscale techniques for the visualization and quantification of cell machinery in real-time and on living cells. This will lead to detailed, quantitative models of sub-cellular structures and molecular complexes under different conditions for both normal and diseased cells.
This approach ultimately requires the development of novel biophysical methods. We have recently pioneered the development of an array of new and powerful biophysical tools based on Scanning Ion Conductance Microscopy [1, 2] that allow quantitative measurements and non-invasive functional imaging of single protein molecules in living cells fig. 1 [3]. Scanning ion conductance microscopy and a battery of associated innovative methods are unique among current imaging techniques, not only in spatial resolution of living and functioning cells fig. 2 [4], but also in the rich combination of imaging with other functional and dynamical interrogation methods (e.g electrophysiological recording from presynaptic boutons fig. 2 [5]). These methods, crucially, will facilitate the study of integrated nano-behaviour in living cells in health and disease.
[1] P. K. Hansma, B. Drake, O. Marti, S. A. Gould, C. B. Prater, Science 243, 641 (1989).
[2] Y. E. Korchev, C. L. Bashford, M. Milovanovic, I. Vodyanoy, M. J. Lab, Biophys. J. 73, 653 (1997).
[3] A. I. Shevchuk et al., Angew. Chem. Int. Ed Engl. 45, 2212 (2006).
[4] P. Novak et al., Nat. Methods 6, 279 (2009).
[5] P Novak, et al., Neuron,79, 1067 (2013)
Bio: Prof Yuri Korchev gained a BSc in Biology & Physiology, a Diploma in University Teaching, and an MSc in Physiology, at the University of St Petersburg, and a PhD (biophysics of ion transport) at the Russian Academy of Sciences. From 1984, he held the post of Research Scientist at the Institute of Cytology, Russian Academy of Sciences, St Petersburg. In 1991 he joined St George's Hospital Medical School, London as a Wellcome Trust Research Fellow and then as a Post-Doctoral Research Fellow. In 1995 he moved to Charing Cross & Westminster Medical School as a Post-Doctoral Research Fellow, and in 1997 became a lecturer at Imperial College School of Medicine, Division of Medicine, Hammersmith Campus. In 2003, Yuri was appointed as Reader and in 2005 as Professor of Biophysics. He now heads the Nanomedicine laboratory at Imperial College London.Yuri is non-executive Chief Scientist of Ionoscope limited.
This approach ultimately requires the development of novel biophysical methods. We have recently pioneered the development of an array of new and powerful biophysical tools based on Scanning Ion Conductance Microscopy [1, 2] that allow quantitative measurements and non-invasive functional imaging of single protein molecules in living cells fig. 1 [3]. Scanning ion conductance microscopy and a battery of associated innovative methods are unique among current imaging techniques, not only in spatial resolution of living and functioning cells fig. 2 [4], but also in the rich combination of imaging with other functional and dynamical interrogation methods (e.g electrophysiological recording from presynaptic boutons fig. 2 [5]). These methods, crucially, will facilitate the study of integrated nano-behaviour in living cells in health and disease.
[1] P. K. Hansma, B. Drake, O. Marti, S. A. Gould, C. B. Prater, Science 243, 641 (1989).
[2] Y. E. Korchev, C. L. Bashford, M. Milovanovic, I. Vodyanoy, M. J. Lab, Biophys. J. 73, 653 (1997).
[3] A. I. Shevchuk et al., Angew. Chem. Int. Ed Engl. 45, 2212 (2006).
[4] P. Novak et al., Nat. Methods 6, 279 (2009).
[5] P Novak, et al., Neuron,79, 1067 (2013)
Bio: Prof Yuri Korchev gained a BSc in Biology & Physiology, a Diploma in University Teaching, and an MSc in Physiology, at the University of St Petersburg, and a PhD (biophysics of ion transport) at the Russian Academy of Sciences. From 1984, he held the post of Research Scientist at the Institute of Cytology, Russian Academy of Sciences, St Petersburg. In 1991 he joined St George's Hospital Medical School, London as a Wellcome Trust Research Fellow and then as a Post-Doctoral Research Fellow. In 1995 he moved to Charing Cross & Westminster Medical School as a Post-Doctoral Research Fellow, and in 1997 became a lecturer at Imperial College School of Medicine, Division of Medicine, Hammersmith Campus. In 2003, Yuri was appointed as Reader and in 2005 as Professor of Biophysics. He now heads the Nanomedicine laboratory at Imperial College London.Yuri is non-executive Chief Scientist of Ionoscope limited.
Practical information
- General public
- Free
Organizer
- Georg Fantner
Contact
- Georg Fantner