Super-resolution by Structured Illumination
Event details
| Date | 12.09.2012 |
| Hour | 09:15 |
| Speaker | Dr. Hesper Rego, Harvard School of Public Health |
| Location | |
| Category | Conferences - Seminars |
Structured-illumination Microscopy (SIM) is a super-resolution technique that relies on patterned illumination to move high-resolution information into the normal pass-band of a fluorescence microscope. SIM can double the resolution of a light microscope using conventional fluorescence, and can achieve theoretically unlimited resolution if a nonlinear fluorescence phenomenon is exploited. Here I will briefly discuss the theory and practical design considerations of SIM. I will then show examples of fixed and live-cell SIM in two and three dimensions and in multiple colors. Finally, I will discuss our work to improve nonlinear SIM such that it is a biologically compatible super-resolution method. Using fluorescence photoswitching, an inherently nonlinear phenomenon, we have reached 40-nm resolution with low light intensities and needing 1000-fold fewer images than localization-based super-resolution methods.
Practical information
- General public
- Free
Organizer
- Seitz Arne <[email protected]>
Contact
- Seitz Arne <[email protected]>