Accelerating protein engineering with viral-based in vivo screening and evolution technologies - CH636
Event details
| Date | 06.09.2024 |
| Hour | 16:00 › 17:00 |
| Speaker | Prof. Bryan Dickinson |
| Location | |
| Category | Conferences - Seminars |
| Event Language | English |
Abstract :
Proteins that selectively bind to a target protein of interest are foundational components of research pipelines, diagnostics, and therapeutics. However, current immunization-based, display-based, and computational approaches for discovering binders are laborious and time-consuming, suffer from high false positives, and have a high failure rate. In this presentation, I will showcase Protein-Protein Interaction Phage-Assisted Non-Continuous Selection (PPI-PANCS), a system for comprehensive high-throughput screening of billion+ member protein variant libraries to isolate high affinity binders to a protein in a matter of days. We demonstrate the utility of PPI-PANCS by screening multiple protein libraries against a panel of 95 targets and generating dozens of novel binders, which can then be affinity matured and/or used in secondary assays, such as triggering degradation of endogenous mammalian proteins.
Biography:
Bryan Dickinson earned his B.S. in Biochemistry from the University of Maryland in 2005, and his Ph.D. in Chemistry in 2010 from the University of California at Berkeley for work performed under the supervision of Professor Christopher Chang. He then moved to Harvard University as a Jane Coffin Childs Memorial postdoctoral fellow under the supervision of Professor David Liu. He joined the faculty at the University of Chicago in the Department of Chemistry in the Summer of 2014, was promoted to Associate Professor in 2019, and Professor in 2023.
The Dickinson Group employs synthetic organic chemistry, molecular evolution, and protein design to develop molecular technologies to study and control chemistry in living systems. The group's current primary research interests include: 1) developing new evolution technologies to reprogram and control biomolecular interactions, 2) engineering RNA-targeting biotechnologies as new therapeutic platforms, and 3) developing novel proximity-labeling chemistries to study biomolecular interactions. Bryan has been recognized by multiple prizes and awards, including the Sloan Foundation Research Fellowship, NSF CAREER Award, Camille Dreyfus Teacher-Scholar Award, and ACS Chemical Biology Young Investigator Award, and Bryan was named an Investigator of the Chan Zuckerberg Biohub this year.
Lab Website:
https://www.dickinsonlab.uchicago.edu
Proteins that selectively bind to a target protein of interest are foundational components of research pipelines, diagnostics, and therapeutics. However, current immunization-based, display-based, and computational approaches for discovering binders are laborious and time-consuming, suffer from high false positives, and have a high failure rate. In this presentation, I will showcase Protein-Protein Interaction Phage-Assisted Non-Continuous Selection (PPI-PANCS), a system for comprehensive high-throughput screening of billion+ member protein variant libraries to isolate high affinity binders to a protein in a matter of days. We demonstrate the utility of PPI-PANCS by screening multiple protein libraries against a panel of 95 targets and generating dozens of novel binders, which can then be affinity matured and/or used in secondary assays, such as triggering degradation of endogenous mammalian proteins.
Biography:
Bryan Dickinson earned his B.S. in Biochemistry from the University of Maryland in 2005, and his Ph.D. in Chemistry in 2010 from the University of California at Berkeley for work performed under the supervision of Professor Christopher Chang. He then moved to Harvard University as a Jane Coffin Childs Memorial postdoctoral fellow under the supervision of Professor David Liu. He joined the faculty at the University of Chicago in the Department of Chemistry in the Summer of 2014, was promoted to Associate Professor in 2019, and Professor in 2023.
The Dickinson Group employs synthetic organic chemistry, molecular evolution, and protein design to develop molecular technologies to study and control chemistry in living systems. The group's current primary research interests include: 1) developing new evolution technologies to reprogram and control biomolecular interactions, 2) engineering RNA-targeting biotechnologies as new therapeutic platforms, and 3) developing novel proximity-labeling chemistries to study biomolecular interactions. Bryan has been recognized by multiple prizes and awards, including the Sloan Foundation Research Fellowship, NSF CAREER Award, Camille Dreyfus Teacher-Scholar Award, and ACS Chemical Biology Young Investigator Award, and Bryan was named an Investigator of the Chan Zuckerberg Biohub this year.
Lab Website:
https://www.dickinsonlab.uchicago.edu
Practical information
- Informed public
- Free